亚临界流体1,1,1,2-四氟乙烷(R134a)萃取黑果枸杞原花青素的工艺研究及不同产地含量测定  

The Technical Research of Extraction of Procyanidins in Lycium ruthenicum Murr. Using Sub-Critical Fluid 1,1,1,2-Tetrafluoroethane (R134a) and the Contents Determination from Different Origins

作　　者：谭亮[1] 朱力 冯志豪 冀恬[1] 迟晓峰[1] 董琦[1] 李玉林[1] 

机构地区：[1]中国科学院西北高原生物研究所、中国科学院藏药研究重点实验室,青海西宁 [2]广州市浩立生物科技有限公司,广东广州

出　　处：《植物学研究》2017年第5期304-312,共9页Botanical Research

基　　金：中国科学院个性化药物先导科技专项资助(XDA12050304);青海省自然科学基金(2015-ZJ-908);青海无公害农产品分析检测平台建设(2015-ZL-Y23);兰州区域中心2016年度仪器功能开发项目:多通道可回收型中试亚临界流体萃取装置的改造(2018gl11)。

摘　　要：在1 L萃取釜中,对亚临界流体1,1,1,2-四氟乙烷(R134a)萃取黑果枸杞中原花青素的最佳工艺进行了研究。以原花青素的萃取率为指标,采用单因素试验和正交试验考察了萃取压力、萃取温度、萃取时间对萃取率的影响。影响黑果枸杞中原花青素提取率的各因素主次顺序为:萃取温度 >萃取压力 >萃取时间,在萃取压力1.2 MPa,萃取温度50℃,萃取时间90 min为最佳提取工艺条件下,黑果枸杞原花青素的萃取率最高。同时在该优化条件下采用香草醛–盐酸法测定了不同产地黑果枸杞中原花青素的含量。该方法易于操作、选择性好、萃取温度低、萃取效率高,适用于黑果枸杞中原花青素的提取。The optimal conditions for extracting procyanidins from Lycium ruthenicum Murr. with sub-cri- tical fluid R134a (1,1,1,2-tetrafluoroethane) in a 1 L extraction vessel were studied. Taking the extraction ratio of procyanidins as an indicator, the influences of pressure, temperature, and extraction time on extraction rate of procyanidins from Lycium ruthenicum Murr. were studied by single factor experimental methods and orthogonal array design. The order of factors affecting extraction ratio of procyanidins was extraction temperature >extraction pressure >extraction time. The optimum extraction conditions were as follows: the extraction ratio of procyanidins from Lycium ruthenicum Murr. was the highest with a extraction pressure of 1.2 MPa, extraction temperature of 50?C and extraction time of 90 min. While the contents of procyanidins in Lycium ruthenicum Murr. from different origins were determined by vanillin-hydrochloric acid method under the optimal conditions. The method has the advantages of easy operation, good selectivity, low extraction temperature and high extraction efficiency, which is suitable for extraction of procyanidins components in Lycium ruthenicum Murr.

关 键 词：黑果枸杞 原花青素 亚临界萃取技术 R134A 香草醛–盐酸法 不同产地 含量测定 

分 类 号：R28[医药卫生—中药学]