拟南芥AtGSTZ1基因启动子与GUS重组载体的构建及转化  

Construction and Transformation of Recombinant Vector Consisting of GUS Gene and Zeta Class Glutathione S-Transferase 1 Promoter in Arabidopsis thaliana

作　　者：蒋嘉彦 董文 黄崇瑞 彭怡 黄丽华[1] 胡超[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙 [2]湖南农业大学国际学院,湖南长沙

出　　处：《植物学研究》2019年第2期158-164,共7页Botanical Research

基　　金：湖南农业大学创新性实验计划项目XCX17066和XCX18102支助。

摘　　要：在植物中,根据序列的同源性将谷胱甘肽转移酶(Glutathione S-transferases, GSTs)分为不同的种类。这些不同种类的谷胱甘肽转移酶在植物生长和发育中发挥着不同的功能。相对于其他种类的谷胱甘肽转移酶,Zeta类谷胱甘肽转移酶(GSTZ)的功能尚需进一步鉴定。本研究克隆了拟南芥AtGSTZ1基因启动子,并将该启动子与GUS表达载体重组。重组载体采用农杆菌介导法转化拟南芥。GUS组织化学染色结果表明,AtGSTZ1基因启动子驱动了GUS基因在拟南芥中表达。实验结果为进一步研究AtGSTZ1的表达特征奠定了基础。Based on the homology comparison,glutathione transferases(GSTs)in plants are distributed into different classes,which are known to play different roles in plant growth and development.Among GSTs,the function of zeta class GSTs(GSTZ)is not well characterized.Here,the promoter sequence of AtGSTZ1 gene was isolated from Arabidopsis,and a fusion expression vector of AtGSTZ1 promoter and GUS gene was constructed and introduced into Arabidopsis by A.tumefaciens-mediated transformation.Histochemical staining of Arabidopsis indicated the AtGSTZ1 promoter drove the GUS expression in Arabidopsis.The results lay the foundation for analyzing expression pattern of AtGSTZ1.

关 键 词：谷胱甘肽转移酶 AtGSTZ1基因 启动子 GUS 

分 类 号：R73[医药卫生—肿瘤]