利用响应面法摸索薄皮甜瓜采后病害生防菌株发酵最佳培养基  

Using Response Surface Methodology to Explore the Best Fermentation Medium for Biocontrol Strain for Post-Harvest Diseases of Melon

作　　者：曹旭[1,2] 商亮 陈静宇[1] 孟利强[1,2] 李晶[1,2] 

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江 哈尔滨 [2]黑龙江省科学院高技术研究院,黑龙江 哈尔滨

出　　处：《农业科学》2020年第8期571-579,共9页Hans Journal of Agricultural Sciences

摘　　要：目的:研究对薄皮甜瓜采后病害具有防治作用的生防菌株浅黄假单胞菌的实验室发酵最佳培养基,为微生物农药应用开发提供优质资源。方法:采用Design-Expert 8.0软件的Plackett-Burman及Box- Behnken设计试验。结果:确定了对引起薄皮甜瓜采后病害主要病原真菌具有拮抗作用的生防菌株浅黄假单孢菌(Pseudomonas lutea)实验室发酵的最佳培养基为:葡萄糖20 g/L,MgSO4 0.2 g/L,牛肉膏8.3 g/L,酵母膏6.9 g/L,K2HPO4 1 g/L,CaCO3 1 g/L。Aim: To study the best culture medium for laboratory fermentation of the biocontrol strain Pseudomonas lutea, which has a preventive effect on the postharvest diseases of muskmelon, and then provide high-quality resources for the application and development of microbial pesticides. Methods: Plackett-Burman and Box-Behnken design experiments using Design-Expert 8.0 software. Results: The optimal medium for laboratory fermentation of the biocontrol strain Pseudomonas lutea, which has antagonistic effect on the main pathogenic fungi that cause the postharvest diseases of muskmelon, was determined as: Glucose 20 g/L, MgSO4 0.2 g/L, beef extract 8.3 g/L, yeast extract 6.9 g/L, K2HPO4 1 g/L, CaCO3 1 g/L.

关 键 词：发酵 浅黄假单孢菌 响应面 

分 类 号：S65[农业科学—果树学]