草菇液体菌种培养条件的对比试验  

Comparative Experiment on the Cultivation Conditions of Volvariella volvacea Liquid Strains

作　　者：覃宝山 赵媛娟[4] 蒙明月 覃勇荣 

机构地区：[1]河池学院化学与生物工程学院,广西 河池 [2]河池学院广西蚕桑生态学与智能化技术应用重点实验室,广西 河池 [3]河池学院广西现代蚕桑丝绸协同创新中心,广西 河池 [4]广西合山市农村农业局,广西 来宾

出　　处：《农业科学》2024年第9期963-970,共8页Hans Journal of Agricultural Sciences

摘　　要：为了筛选合适的草菇液体菌种培养条件,本实验设计三个配方进行草菇液体菌种摇床培养对比试验,筛选出适宜的培养基配方,即马铃薯50 g,葡萄糖20 g,蛋白胨1.5 g,KH2PO4 1 g,MgSO4 0.5 g,维生素B1 10 mg,水1000 mL,pH自然。在此基础上设计摇床转速、维生素B1添加浓度、培养基装瓶量、摇前静置时间等四个因子,进行草菇液体菌种摇床培养的比较试验。结果表明,草菇液体菌种培养最适合的摇床转速是130 r/min;添加的维生素B1的最佳浓度为15 mg/L;规格为250 mL的锥形瓶,培养基的最适装瓶量为80 mL;培养基静置36 h再进行摇床培养效果最好。In order to screen suitable culture conditions of liquid strains for Volvariella volvacea, this experiment designed three formulas for comparative shaking table cultivation of V. volvacea liquid strains. The suitable medium formula was selected, namely 50 g of potato, 20 g of glucose, 1.5 g of peptone, 1 g of KH2PO4, 0.5 g of MgSO4, 10 mg of Vitamin B1, 1000 mL of water, and natural pH. On this basis, four factors were designed, including shaking table speed, vitamin B1 addition concentration, bottling volume of culture medium, and settling time before shaking, to conduct comparative experiments on V. volvacea liquid strain shaking table cultivation. The results showed that the most suitable shaking table speed for the cultivation of V. volvacea liquid strains was 130 r/min;the optimal concentration of added vitamin B1 was 15 mg/L;the optimal filling volume for a 250 mL conical flask and culture medium was 80 mL;the best effect was to let the culture medium stand for 36 hours before shaking it on a shaker.

关 键 词：草菇 液体菌种 摇床培养 菌丝生长 

分 类 号：S64[农业科学—蔬菜学]