BnaHKL1基因过量表达与转基因油菜叶片中可溶性糖的变化  

Overexpression of the BnaHKL1 Gene and Changes in Soluble Sugars in the Leaf Tissues of Transgenic Rapeseed

作　　者：刘佳桢 钟微 杨慧[1,2] 田闵玉 阮颖 刘春林[1,2] 

机构地区：[1]湖南农业大学,作物表观遗传调控与发育湖南省重点实验室,湖南 长沙 [2]岳麓山实验室,湖南 长沙

出　　处：《农业科学》2024年第12期1307-1315,共9页Hans Journal of Agricultural Sciences

基　　金：国家自然基金委联合重点项目(U20A2028),湖南农业大学研究生科研创新项目(2023XC078)。

摘　　要：本研究构建了甘蓝型油菜BnaHKL1基因的过量表达载体pFGC5941-BnaHKL1,并采用根癌农杆菌介导的方法对甘蓝型油菜湘油15 (XY15)的下胚轴外植体进行转化。通过PCR鉴定,成功获得34株抗性植株,其中11株是具有基因过量表达的转基因植株。半定量RT-PCR分析结果表明,过表达转基因植株中BnaHKL1基因的表达水平显著高于XY15中的表达水平。与XY15相比,BnaHKL1过表达植株的淀粉含量显著降低,而蔗糖、可溶性糖和果聚糖的含量则显著升高。In this study, the overexpression vector pFGC5941-BnaHKL1 of the BnaHKL1 gene from Brassica napus was constructed, and the hypocotyl explants of Brassica napus Xiangyou 15 (XY15) were transformed by the Agrobacterium tumefaciens-mediated method. Through PCR identification, 34 resistant positive plants were successfully obtained, among which 11 were transgenic plants with overexpression cassette of the BnaHKL1 gene. The results of semi-quantitative RT-PCR analysis indicated that the expression level of the BnaHKL1 gene in the overexpression transgenic plants was significantly higher than that in XY15. Compared with XY15, the starch content in BnaHKL1 overexpression plants was significantly decreased, whereas the contents of sucrose, soluble sugars, and fructan were significantly increased.

关 键 词：HKL1 过量表达 转基因植株 可溶性糖 

分 类 号：R73[医药卫生—肿瘤]