欧李凝集素对微藻、细菌和酵母凝集作用分析  

Analysis of Agglutination from Cerasus humilis Lectin on Micro Algae, Bacteria and Yeast

作　　者：韩红艳[1] 冀爱青[1] 陈林晶[1] 杜俊杰[2] 

机构地区：[1]晋中学院,山西晋中 [2]山西农业大学,山西晋中

出　　处：《生物医学》2017年第2期25-30,共6页Hans Journal of Biomedicine

基　　金：晋中市软科学计划项目(项目编号:R1601)。

摘　　要：本试验以欧李果仁为材料,经石油醚脱脂后,用磷酸盐缓冲液PBS (PH7.4)浸提,50%饱和硫酸铵沉淀,PBS透析,获得欧李凝集素粗提液,分别研究它对微藻、细菌和酵母的凝集作用。研究结果表明:欧李凝集素能够凝集铜绿微囊藻和斜生栅藻,对蛋白核小球藻无凝集作用;对大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌均有一定的凝集能力,并且随细菌密度的增加,凝集现象越明显;欧李凝集素能凝集热处理后的酵母菌细胞,凝集素浓度越高,凝集效果越显著。A lectin has been isolated from seed of the Cerasus humilis using a procedure that involved de-greasing of petroleum ether, extraction of phosphate buffer, precipitation of 50% ammonium sulfate and dialysis with PBS. Then we researched on its agglutination activity toward micro algae, bacteria and yeast. The results showed that Cerasus humilis lectin could not agglutinate Chlorella pyrenoidosa of freshwater micro algae, but could agglutinate Microcystis aeruginosa and Scenedesmus obliquus;Cerasus humilis lectin had ability to agglutinate Escherichia coli, Bacillus subtilis and Staphylococcus aureus. As the bacterial density increased, agglutination became more and more obvious;Cerasus humilis lectin could agglutinate yeast cells after heat treatment. In general, the higher the concentration of lectin is, the greater the effect of agglutination will be.

关 键 词：欧李凝集素 凝集作用 微藻 细菌 酵母 

分 类 号：R28[医药卫生—中药学]