3D打印乳腺癌模型及白藜芦醇与华蟾素药敏试验  

3D Bioprinted Breast Cancer Model and Its Application in Drug Sensitivity Test of Resveratrol and Cinobufagin

作　　者：王晗 朱莲 唐靓 杨亚冬 赵思雨 张文元 

机构地区：[1]杭州医学院公共卫生学院,浙江 杭州 [2]杭州医学院基础医学与法医学院,浙江 杭州 [3]杭州医学院检验医学院、生物工程学院,浙江 杭州

出　　处：《生物医学》2023年第4期359-367,共9页Hans Journal of Biomedicine

摘　　要：目的:探讨三维(3D)生物打印MDA-MB-231乳腺癌细胞模型及其用于2种中药白藜芦醇与华蟾素药敏试验的可行性。方法:通过3D生物打印构建3D乳腺癌模型,采用该模型进行白藜芦醇、华蟾素药敏试验,并与二维(2D)培养比较。结果:在3D/2D培养下,随着这2种中药浓度的升高,乳腺癌细胞存活率成下降趋势;这2种中药在3D培养下对乳腺癌细胞的药敏性均显著低于2D培养。结论:使用3D生物打印技术构建的3D乳腺癌模型,可能更好地模拟体内乳腺癌肿瘤微环境,以提高临床前药物的预测能力,改善药物临床转化。Objective: To explore the feasibility of 3D bioprinted model of MDA-MB-231 breast cancer cells and its application in drug sensitivity test of resveratrol and cinobufagin. Methods: The 3D model of breast cancer was constructed by 3D bioprinting, then the model was used to test the drug sensitivity of resveratrol and cinbufosinthe, and the results were compared with those under 2D culture conditions. Results: The results showed that, in 3D/2D culture, the survival rate of breast cancer cells decreased with the increase of the concentrations of resveratrol and cinobufagin. The drug sensitivities of resveratrol and cinobufagin to breast cancer cells in 3D culture was significantly lower than those in 2D culture. Conclusion: The results indicate that the breast cancer model constructed by 3D bioprinting technology may better simulate the microenvironment of breast cancer in vivo, so as to improve the prediction ability of preclinical drug research and the clinical transformation of drugs.

关 键 词：3D生物打印 3D乳腺癌模型 白藜芦醇 华蟾素 药敏试验 

分 类 号：R73[医药卫生—肿瘤]