直接巢式PCR结合测序检测5-HT1A基因C-1019G多态性的方法的建立  

Establishment of Direct Nested PCR Combined with Sequencing for Detection of C-1019G Polymorphism in 5-HT1A Gene

作　　者：王川均 杨玉娟 何震宇[3] 潘伟 

机构地区：[1]广东药科大学临床药学院,广东 广州 [2]广东药科大学基础医学院,广东 广州 [3]广东药科大学学生工作部,广东 广州

出　　处：《生物医学》2024年第4期645-651,共7页Hans Journal of Biomedicine

摘　　要：目的:5-HT1A基因C-1019G多态性与多种神经精神疾病如抑郁症、精神分裂症、焦虑症等的发生风险、症状严重程度及治疗效果存在关联。本研究旨在建立一种直接巢式PCR结合测序检测该位点的方法。方法:以口腔上皮细胞粗处理物为材料,通过直接巢式PCR扩增包含5-HT1A基因C-1019G位点的靶片段,PCR产物经桑格测序鉴定基因型。结果:所检样本均能扩增出预期大小的PCR产物,测序峰图清晰。结论:成功建立了一种直接巢式PCR结合测序鉴定5-HT1A基因型的方法,有良好的应用前景。Objective: The C-1019G polymorphism of the 5-HT1A gene has been associated with the risk of occurrence, symptom severity, and treatment outcome of a variety of neuropsychiatric disorders, such as depression, schizophrenia, and anxiety disorders. The aim of this study was to establish a direct nested PCR combined with sequencing to detect this locus. Methods: The target fragment containing the C-1019G locus of the 5-HT1A gene was amplified by direct nested PCR using crude processed oral epithelial cells, and the PCR product was genotyped by Sanger sequencing. Results: PCR products of expected size were amplified from all the samples tested, and the sequencing peaks were clear. Conclusion: A direct nested PCR combined with sequencing method was successfully established to identify the genotypes of 5-HT1A gene, which has good prospects for application.

关 键 词：5-HT1A基因 C-1019G多态性 直接巢式PCR 测序 

分 类 号：R74[医药卫生—神经病学与精神病学]