喘可治注射液对人胚胎肺成纤维细胞转化因子及相关蛋白的影响  

Effect of Chuankezhi Injection on Transfer Factors and Related Proteins of Human Embryonic Lung Fibroblasts

作　　者：闫红倩[1,2] 耿立梅 于向艳[1,2] 马蕴蕾 

机构地区：[1]河北省中医院,河北 石家庄 [2]河北省中西医结合肺病研究重点实验室,河北 石家庄

出　　处：《药物化学》2021年第2期78-86,共9页Hans Journal of Medicinal Chemistry

摘　　要：目的:通过喘可治注射液(CKZ)干预人胚胎肺成纤维细胞(Wi-38),测定β转化生长因子(TGF-β)、1型胶原纤维蛋白(collagen1)和α-平滑肌肌动蛋白(α-SMA),探讨CKZ在抑制细胞间质转化方面的作用机制。方法:将体外培养的Wi-38细胞随机分为6组:① 对照组,单独的DMEM培养;② 5 ng/mL TGF-β组,加入浓度为5 ng/mL的TGF-β孵育;③ 10 ng/mL TGF-β组,加入浓度为10 ng/mL的TGF-β孵育;④10 ul/mL喘可治注射液(CKZ)组,加入浓度为10 ul/mL CKZ的孵育;⑤ 20 ul/mL CKZ组,加入浓度为20 ul/mL CKZ的孵育;⑥ 10 ng/mL TGF-β + 20 ul/mL CKZ组,同时加入浓度10 ng/mL TGF-β和20 ul/mL CKZ孵育。采用实时荧光定量(qPCR)的方法检测孵育24 h、48 h、72 h的TGF-β、collagen 1、α-SMA的表达水平变化。结果:加入TGF-β处理组细胞的α-SMA、Collagen 1的mRNA水平升高、TGF-β的mRNA水平降低,与对照组相比,其差异均具有统计学意义(P 【0.05);加入CKZ处理组细胞的α-SMA、TGF-β、Collagen 1的mRNA水平均降低,与对照组相比,其差异具有统计学意义(P 【0.05);同时加入TGF-β和CKZ处理组细胞的α-SMA、Collagen 1的mRNA水平均显著降低,与TGF-β处理组相比,其差异具有统计学意义(P 【0.05)。结论:TGF-β、collagen 1、α-SMA参与了人胚胎肺成纤维细胞间质转化的病理进程,CKZ对这一进程起到了抑制作用,CKZ具有一定的抑制气道上皮间质转化的作用。Objective: To investigate the effect of Chuankezhi injection (CKZ) on human embryonic lung fibro-blasts (WI-38), and to explore the mechanism of CKZ in inhibiting the mesenchymal transition by measuring the levels of TGF-β, collagen 1 and α-smooth muscle actin (α-SMA). Method: The cultured Wi-38 cells were randomly divided into 6 groups: ① control group, DMEM incubatedalone;② 5 ng/mL TGF-β group, incubated with 5 ng/mL TGF-β;③ 10 ng/mL TGF-β group, incubated with 10 ng/ml TGF-β;④ 10 ul/mL CKZ group, incubated with 10 ul/mL CKZ;⑤ 20 ul/mL CKZ group, in-cubated with 20 ul/mL CKZ;⑥ 10 ng/mL TGF-β + 20 ul/mL CKZ group, incubated with 10 ng/mL TGF-β and 20 ul/mL CKZ. The expression levels of TGF-β, collagen 1 and α-SMA were detected by qPCR at 24, 48 and 72 h. Result: Compared with the control group, the mRNA levels of α - SMA and collagen 1 were increased and the mRNA levels of TGF-β were decreased in the TGF-β treated group (P <0.05). The mRNA levels of α-SMA, TGF-β and collagen 1 in CKZ treated cells were all significantly decreased (P <0.05);Compared with the TGF-β treated group, the mRNA levels of α-SMA and colla-gen 1 were decreased in the both TGF-β and CKZ treated groups (P <0.05). Conclusion: TGF-β, colla-gen 1 and α-SMA participate in the pathological process of mesenchymal transition of human em-bryonic lung fibroblasts. CKZ may inhibit this process, and CKZ may inhibit airway epithelial mes-enchymal transition to some extent.

关 键 词：喘可治注射液 Wi-38 TGF-Β Α-SMA Collagen 1 气道上皮间质转化 

分 类 号：R73[医药卫生—肿瘤]