电针对兔激素性股骨头坏死血清HIF-1α、VEGF、BMP2、Osterix的影响  

Effect of Electroacupuncture on Serum HIF-1α, VEGF, BMP2, and Osterix in Rabbits with Steroid-Induced Femoral Head Necrosis

作　　者：宋红梅[1] 廖林 谢文博 林菲菲 王和鸣[4] 

机构地区：[1]福建中医药大学附属第二人民医院推拿科,福建 福州 [2]福建中医药大学针灸推拿学院,福建 福州 [3]福州市长乐区中医院康复科,福建 福州 [4]福建中医药大学骨伤学院,福建 福州

出　　处：《临床个性化医学》2024年第4期2691-2696,共6页Journal of Clinical Personalized Medicine

基　　金：国家自然科学基金项目(81973879);福建省财政厅卫生健康省级补助资金(C2023003-财政专项)。

摘　　要：目的:观察电针对兔激素性股骨头坏死血清中成血管因子HIF-1α、VEGF;成骨因子BMP2、Osterix;H型血管标志物CD31;骨破坏相关因子MMP13的影响,探索电针治疗激素性股骨头坏死的治疗作用及机制。方法:选取健康雄性新西兰大白兔18只,随机分成正常组、模型组、电针组(电针环跳穴、髀关穴),每组6只。运用qRT-PCR试剂盒检测兔血清中HIF-1α、VEGF、BMP2、Osterix含量。运用免疫荧光染色检测H型血管标志物CD31、骨破坏相关因子MMP13表达情况。结果:HIF-1α、VEGF、BMP2、Osterix的含量,模型组浓度明显低于正常组(P α、BMP2、Osterix、CD31的表达,从而造成骨质破坏和血管破坏,导致股骨头坏死。电针能调控成血管相关因子VEGF、HIF-α,成骨相关因子BMP2、Osterix,H型血管标志物CD31,使VEGF、HIF-α、BMP2、Osterix、CD31浓度均增高,促进股骨头内血管新生,骨破坏相关因子MMP13表达量下降,从而增强骨组织自身修复能力。这可能是电针治疗激素性股骨头坏死的机制之一。Objective: To observe the effect of electroacupuncture on angiogenic factors HIF-1α and VEGF;osteogenic factors BMP2 and Osterix;H-type vascular marker CD31;and bone destruction-related factor MMP13 in the serum of rabbits with steroid-induced femoral head necrosis, and to explore the therapeutic effect and mechanism of electroacupuncture in the treatment of steroid-induced femoral head necrosis. Methods: Eighteen healthy male New Zealand white rabbits were randomly divided into a normal group, a model group, and an electroacupuncture group (electroacupuncture at Huantiao acupoint and Biguan acupoint), with 6 rabbits in each group. The levels of HIF-1α, VEGF, BMP2, and Osterix in rabbit serum were detected by qRT-PCR kit. Immunofluorescence staining was used to detect the expression of H-type vascular marker CD31 and bone destruction-related factor MMP13. Results: The concentrations of HIF-1α, VEGF, BMP2, and Osterix in the model group were s

关 键 词：激素性股骨头坏死 电针 成血管相关因子VEGF、HIF-α 成骨相关因子BMP2、Osterix H型血管标志物CD31 骨破坏相关因子MMP13 

分 类 号：R73[医药卫生—肿瘤]