柴胡皂苷d与阿奇霉素抗耐药支原体活性的对比研究  

A Comparative Study on the Activity of Saikosaponin d and Azithromycin against Mycoplasma

作　　者：贾亚利[1] 刘珏[2] 吴黎明[2] 蔡莺莲[1] 吴盛海[2] 

机构地区：[1]杭州市肿瘤医院皮肤科,浙江杭州 [2]杭州市第一人民医院皮肤科,浙江杭州

出　　处：《医学诊断》2018年第3期49-54,共6页Medical Diagnosis

摘　　要：目的:检测柴胡中具有抗菌活性的柴胡皂苷单体d (Sd),研究该单体对支原体阿奇霉素耐药株的抑菌作用及其与阿奇霉素的相互作用。方法:采用棋盘微量稀释法,测定单用柴胡皂苷d,阿奇霉素及二者联用使用时,对20株阿奇霉素耐药支原体菌株的最低抑菌浓度MIC,计算部分抑菌浓度指数(FIC),判定二药相互作用。结果:单独用药时Sd及阿奇霉素,对耐阿奇霉素支原体株MIC的几何均数值GM值分别为1.008 ug/mL、1.941 ug/mL。联合用药时,Sd和阿奇霉素的GM值分别降低为0.346 ug/mL和1.189ug/mL。2种药物单用和联合用药时,MIC值差异均有统计学意义(P <0.05)。联用时在20株耐药株均表现为协同或者相加作用。结论:柴胡皂苷单体Sd对解脲支原体耐阿奇霉素菌株有较强的抑制作用,与阿奇霉素有协同或者相加作用。Objective: To detect the antibacterial activity of Saikosaponin monomer d (Sd) in Radix Bupleuri and to evaluatethe antibacterial activity of Sd against azithromycin-resistant Mycoplasma strains and its interaction with azithromycin. Methods: The minimum inhibitory concentration (MIC) of 20 azithromycin-resistant Mycoplasma strains were determined by the chessboard microdilution method when using Saikosaponin d, azithromycin alone and in combination. Partial antibacterial concentration index (FIC) was calculated and interaction between these two drugs were determined. Results: When used alone, the geometric mean (GM) values of MIC of Sd and azithromycin against azithromycin-resistant Mycobacterium were 1.008 ug/mL and 1.941 ug/mL, respectively. When used in combination, the GM values of Sd and azithromycin decreased to 0.346 ug/mL and 1.189 ug/mL, respectively. The MIC values significantly differ between the two drugs used alone and in combination (P <0.05). When used in combination, the two drugs showed synergistic or additive effect toward 20 resistant strains. Conclusion: Sd of Saikosaponin showed a strong inhibitory effect on azithromycin-resistant Ureaplasma urealyticum strains and has synergistic or additive effect with azithromycin.

关 键 词：柴胡皂苷单体 解脲支原体 阿奇霉素 体外抗菌活性 

分 类 号：R73[医药卫生—肿瘤]