线纹海马的性别特异分子标记的开发及鉴定  

Development and Identification of Sex-Specific Molecular Markers in Hippocampus erectus

作　　者：杨子涵 贾枨茜 孙金辉 马菁菁 范倩茹 杨燕菁 

机构地区：[1]天津农学院水产学院,天津

出　　处：《水产研究》2024年第2期49-55,共7页Open Journal of Fisheries Research

摘　　要：性别特异分子标记的筛选是分子辅助单性育种的重要技术手段。本研究基于雌雄各20尾线纹海马的2b-RAD测序数据,比较分析并筛选出一个雄性特异的tag scaffold63和一个性别二态的SNP位点QSNP63,并在大规模群体中进行了验证。PCR扩增显示在108尾海马中scaffold63 tag仅在雄海马中检测到,而在雌海马中未检测出目的片段;108只海马中QSNP63位点测序显示所有雌性个体中该位点为G/G纯合,所有雄性个体中该位点呈现G/T杂合状态。基因注释发现tag scaffold63位于cilia- and flagella-associated protein 69-like25号外显子后的内含子上,QSNP63位于leucine-rich repeats and IQ motif containing 1基因8号外显子上。综上,本研究筛选到的线纹海马性别特异分子标记及候选基因为进一步解析线纹海马的性别决定机制奠定了基础。Screening of sex-specific molecular markers is an important technique for breeding of mono-sex population. Based on the 2b-RAD sequencing data of 20 males and 20 females of Hippocampus erectus, we screened and verified a male-specific tag scaffold63 and a sex-dimorphic SNP site QSNP63 in a large-scale population. In 108 Hippocampus erectus, scaffold63 tag was only detected in males but not in females via PCR amplification. Sequencing of QSNP63 site showed nsSNPs with homozygous (G/G) in females and heterozygous (G/T) in males. Gene annotation revealed that scaffold63 tag located on the intron behind Exon 25 of cilia- and flagella-associated protein 69-like, QSNP63 located on Exon 8 of the leucine-rich repeats and IQ motif containing 1. In conclusion, the sex-specific molecular markers and candidate genes screened in this study offer novel insights into the sex- determination mechanisms of the Hippocampus erectus.

关 键 词：线纹海马 分子标记 SNP 性别决定 2b-RAD 

分 类 号：R74[医药卫生—神经病学与精神病学]