益肺消瘤颗粒诱导肺癌细胞衰老的机制研究  

Experimental Study on Mechanism of Senescence Induced by Yifeixiaoliu Granules in Lung Cancer Cells

作　　者：魏冬梅[1] 陈宏[1,2] 孙玺媛 姜梅[1] 陈沫岚[1] 刘玲玲 张杰 周旭东 苏悦 

机构地区：[1]齐齐哈尔市第一医院,黑龙江 齐齐哈尔 [2]黑龙江省中医科学院,黑龙江 哈尔滨

出　　处：《中医学》2023年第5期1036-1043,共8页Traditional Chinese Medicine

摘　　要：目的:探讨益肺消瘤颗粒诱导肺癌细胞衰老的机制。方法:应用血清药理学方法,β-半乳糖苷酶染色试剂盒检测药物干预后的人肺腺癌A549细胞发生衰老;免疫荧光法检测人肺腺癌A549细胞的PCNA表达;West-blot、RT-PCR检测p16、p21、RB及p-RB的蛋白及mRNA表达。结果:与空白(血清)对照组比较,益肺消瘤颗粒组衰老细胞的比率为6.85%,有统计学差异(P < 0.05);与空白血清组(18.7%)比较,益肺消瘤颗粒组PCNA (6.6%)的表达显著下降,有统计学差异(P < 0.001);与空白(血清)对照组比较,益肺消瘤颗粒组的p16、p21的蛋白表达高于空白(血清)对照组(P < 0.001),RB、p-RB的蛋白表达低于空白(血清)对照组(P < 0.01),有统计学差异;与空白(血清)对照组比较,益肺消瘤颗粒组的p16、p21的mRNA表达高于空白(血清)对照组(P < 0.001),RB的mRNA表达低于空白(血清)对照组(P < 0.01),有统计学差异。结论:益肺消瘤颗粒通过抑制PCNA的表达、上调p16、p21的蛋白及mRNA的表达,下调RB及p-RB的蛋白及mRNA的表达诱导肺癌细胞衰老。Objective: To investigate the mechanism of senescence induced by Yifeixiaoliu granules in lung cancer cells. Methods: By serum pharmacology, β-galactosidase staining kits were used to detect the senescence of human lung adenocarcinoma A549 cells after drug intervention. The expression of PCNA in human lung adenocarcinoma A549 cells was detected by immunofluorescence. The protein and mRNA expressions of p16, p21, RB and p-RB were detected by West-blot and RT-PCR. Results: Compared with blank (serum) control group, the ratio of senescent cells in Yifeixiaoliu Granules group was 6.85%, with statistical difference (P < 0.05);Compared with blank serum group (18.7%), the expression of PCNA in Yifeixiaoliu granules group (6.6%) was significantly decreased, and the difference was statistically significant (P < 0.001);Compared with blank (serum) control group, the protein expressions of p16 and p21 in Yifeixiaoliu granules group were higher than those in blank (serum) control group (P < 0.001), and the protein expressions of RB and p-Rb were lower than those in blank (serum) control group (P < 0.01), and the difference was statistically significant;Compared with the blank (serum) control group, the mRNA expression of p16 and p21 in YifeiXiaoliu granules group was higher than that in the blank (serum) control group (P < 0.001), and the mRNA expression of RB was lower than that in the blank (serum) control group (P < 0.01), and the difference was statistically significant. Conclusion: Yifeixiaoma granules can induce senescence of lung cancer cells by inhibiting the expression of PCNA, up-regulating the expression of protein and mRNA of p16 and p21, and down-regulating the expression of protein and mRNA of RB and p-RB.

关 键 词：中医药 衰老 肺癌 

分 类 号：R73[医药卫生—肿瘤]