胃癌组织中MGMT、RASSF1A基因启动子区甲基化研究  

The Expression and Promoter Hypermethylation Status of MGMT, RASSF1A in Gastric Cancer

作　　者：鞠昕丽[1] 黄俊谦[2] 宋明全[2] 姜相君[2] 

机构地区：[1]大连医科大学,大连 [2]青岛市市立医院消化内二科,青岛

出　　处：《世界肿瘤研究》2014年第2期18-24,共7页World Journal of Cancer Research

基　　金：青岛市科技局资助项目(2012-1-3-1-(10)-nsh)。

摘　　要：目的:探讨MGMT、RASSF1A启动子甲基化状况在胃癌发生、发展中的作用。方法:用甲基特异性聚合酶扩增链式反应(methylation specific PCR, MSP)检测60例胃癌组织及相应癌旁正常组织MGMT、RASSF1A基因启动子的甲基化状况,用实时荧光定量逆转录–多聚合酶链反应(real-time RT-PCR)和免疫组织化学SP法分别检测基因MGMT、RASSF1A的mRNA和蛋白的表达情况。结果:胃癌组织中MGMT、RASSF1A基因的甲基化阳性率较正常组织明显升高(P <0.05)。胃癌组织中MGMT和RASSF1AmRNA阳性表达率明显低于正常组织(P <0.05)。胃癌组织MGMT、RASSF1A蛋白表达明显低于正常组织。结论:胃癌组织中存在一定程度的MGMT、RASSF1A基因启动子高甲基化和蛋白表达缺失,是胃癌发生的重要途径之一。Objective: To investigate the functions of hypermethylation of MGMT and RASSF1A genes in human gastric cancer. Methods: Methylation status of the MGMT and RASSF1Agenes in 60 gastric carcinoma tissues and 60 paired surgical marginal normal gastric tissues were detected using methylation-specific PCR. Real-time RT-PCR was used to detect the expression of MGMT and RASSF1A, and immunohistochemistry was used to detect the expression of MGMT and RASSF1Aproteins in the above samples. Results: The positive rates of promoter methylation of the MGMT and RASSF1Agenes were significantly higher in gastric cancer than those in normal tissue (both P .

关 键 词：胃癌 甲基化特异性PCR MGMT RASSF1A 

分 类 号：R73[医药卫生—肿瘤]