CARM1通过调控Bax/Bcl-2表达和Caspase-3活性进而调控肝癌生长作用  

CARM1 Regulates the Growth of Hepatocellular Carcinoma Cells via Regulation of Bax/Bcl-2 Level and Caspase-3 Activity

作　　者：刘美玲[1] 洪宏海 王征[1] 

机构地区：[1]广州医科大学附属第三医院检验科,广东广州

出　　处：《世界肿瘤研究》2019年第3期90-97,共8页World Journal of Cancer Research

基　　金：广州医科大学博士启动基金(项目编号:2015C24);广州市卫生局一般引导项目(项目编号:20171A011312);广州市卫生局中西医结合项目(项目编号:20182A011020)广州医科大学大学生实验室开放项目(项目编号:B185004039);广东省教育厅项目(项目编号:2017KTSCX155);广东省自然科学基金(项目编号:2018A030310298)。

摘　　要：目的:共激活因子相关精氨酸甲基转移酶1 (Coactivator-associated arginine methyltransferase 1, CARM1)在肝癌细胞中生长作用及机制研究。方法:qRT-PCR、western blot法检测肝癌细胞中CARM1表达;MTT法和台盼蓝实验检测肝癌细胞的生长情况;Caspase-3试剂盒检测Caspase-3活性;小分子干扰RNA干扰CARM1研究其作用和分子机制。结果:小分子干扰RNA明显敲低CARM1水平;干扰CARM1后明显抑制肝癌细胞生长,且下调抑凋亡蛋白Bcl-2和上调促凋亡蛋白Bax表达水平,同时激活Caspase-3活性。结论:CARM1通过调控Bax/Bcl-2表达和Caspase-3活性进而调控肝癌生长作用。Objective:To investigate the effect and mechanism of CARM1 on the growth of HCC.Methods:qRT-PCR and western blot were used to detect the expression of CARM1.MTT assay and Trypan blue assay were used to detect the growth of hepatocellular carcinoma cells;Caspase-3 kit was used to detect the activity of Caspase-3;small interfering RNA interference CARM1 was used to study its role and molecular mechanism on HCC growth.Results:Small interfering RNA significantly knocked down the level of CARM1;interfering with CARM1 significantly,which inhibited the growth of hepatocellular carcinoma cells,down-regulated Bcl-2,up-regulated Bax expression,and activated Caspase-3 activity.Conclusion:CARM1 regulates the growth of hepatocellular carcinoma by regulating the expression of Bax/Bcl-2 and the activity of Caspase-3.

关 键 词：肝癌 CARM1 BCL-2 BAX CASPASE-3 

分 类 号：R73[医药卫生—肿瘤]