一株腐败希瓦氏菌(Shewanella putrefaciens)培养基优化  

Medium (CM0847) Optimization of Shewanella putrefaciens

作　　者：成娟 王磊 陶榆伟 袁文娟 赵建[1] 冯甦[1] 

机构地区：[1]四川大学生命科学学院资源微生物及生物技术重点实验室,四川成都

出　　处：《水污染及处理》2018年第2期103-109,共7页Water Pollution and Treatment

摘　　要：目的:对发酵培养基(CM0847)进行优化促进腐败希瓦氏菌(Shewanella putrefaciens)生长。方法:通过测定菌体在600 nm处的吸光值和菌体干重,采用单因子试验结合正交试验对培养基(CM0847)进行优化。结果:当pH为7.5,在原培养基的基础上葡萄糖、牛肉膏、氯化钠分别以10%、30%及30%的添加量,菌体密度及菌体干重最高。结论:腐败希瓦氏菌在优化后的培养基中培养,其菌体干重较原培养基(CM0847)增加了11.1168 g/L。Aim: The culture medium of Shewanella putrefaciens was optimized and its growth was promoted. Methods: Single factor and orthogonal design experiment were conducted to determine the optimal medium of Shewanella putrefaciens by measured cell density (under 600 nm) and cell weight. Results: Compared with medium CM0847, when the initial pH was 7.5, the glucose, beef extract and sodium chloride added at 10%, 30% and 30%, respectively, the cell density and cell weight were the highest. Conclusion: Cultivated Shewanella putrefaciens in the optimum medium and CM0847 culture medium, the cells weight was improved by 11.1168 g/L.

关 键 词：SHEWANELLA PUTREFACIENS 优化 培养基 正交 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]