Renewed Focus on the Threat of Cross-Contamination in Molecular Laboratories: Notes from the COVID-19 Testing Experience in Bangladesh  

作　　者：Mohammad Jahidur Rahman Khan Ruksana Raihan S. M. Rashed Ul Islam Nusrat Mannan Mohammad Jamal Uddin Nazia Hasan Khan Tanzila Rawnuck Amirul Huda Bhuiyan Farzana Mim Md. Selim Reza Mohammad Jahidur Rahman Khan;Ruksana Raihan;S. M. Rashed Ul Islam;Nusrat Mannan;Mohammad Jamal Uddin;Nazia Hasan Khan;Tanzila Rawnuck;Amirul Huda Bhuiyan;Farzana Mim;Md. Selim Reza(Department of Microbiology, Shaheed Suhrawardy Medical College, Dhaka, Bangladesh;Department of Microbiology, US-Bangla Medical College, Dhaka, Bangladesh;Department of Virology Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh;Department of Microbiology & Mycology, National Institute of Preventive and Social Medicine, Dhaka, Bangladesh;RT-PCR LAB, United Hospital Limited, Dhaka, Bangladesh;Department of Pathology & Microbiology, Dhaka Dental College, Dhaka, Bangladesh;Department of Virology, Dhaka Medical College, Dhaka, Bangladesh;Department of Biochemistry & Molecular Biology, Jahangirnagar University, Dhaka, Bangladesh;RT-PCR LAB, Bangabandhu Sheikh Mujib Medical College, Faridpur, Bangladesh)

机构地区：[1]Department of Microbiology, Shaheed Suhrawardy Medical College, Dhaka, Bangladesh [2]Department of Microbiology, US-Bangla Medical College, Dhaka, Bangladesh [3]Department of Virology Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh [4]Department of Microbiology & Mycology, National Institute of Preventive and Social Medicine, Dhaka, Bangladesh [5]RT-PCR LAB, United Hospital Limited, Dhaka, Bangladesh [6]Department of Pathology & Microbiology, Dhaka Dental College, Dhaka, Bangladesh [7]Department of Virology, Dhaka Medical College, Dhaka, Bangladesh [8]Department of Biochemistry & Molecular Biology, Jahangirnagar University, Dhaka, Bangladesh [9]RT-PCR LAB, Bangabandhu Sheikh Mujib Medical College, Faridpur, Bangladesh

出　　处：《Advances in Infectious Diseases》2021年第4期357-365,共9页传染病进展（英文）

摘　　要：Rapid and accurate laboratory diagnosis of SARS-CoV-2 infection is crucial for the management of COVID-19 patients and control of the spread of the virus. At the start of the COVID-19 pandemic, Bangladesh had only one government molecular laboratory where real-time RT-PCR would be performed to diagnose SARS-CoV-2 infection. With the increasing number of suspected cases requiring confirmation diagnostic testing, there is a requirement to expand capacity for large-scale testing quickly. The government of Bangladesh established over 100 molecular laboratories within one year to test COVID-19. To expand the testing capacity, the government was compelled to recruit laboratory staff with limited experience and technical expertise, especially in molecular assays, to process specimens, interpret results, troubleshoot. As a result, the risk of diagnostic errors, such as cross-contamination, increased, potentially undermining the efficacy of public health policies, public health response, surveillance programs, and restrictive measures aimed toward containing the outbreak. In this piece, we discuss the different sources of cross-contamination in the COVID-19 RT-PCR laboratories and proffer practical preventive measures to avoid them.Rapid and accurate laboratory diagnosis of SARS-CoV-2 infection is crucial for the management of COVID-19 patients and control of the spread of the virus. At the start of the COVID-19 pandemic, Bangladesh had only one government molecular laboratory where real-time RT-PCR would be performed to diagnose SARS-CoV-2 infection. With the increasing number of suspected cases requiring confirmation diagnostic testing, there is a requirement to expand capacity for large-scale testing quickly. The government of Bangladesh established over 100 molecular laboratories within one year to test COVID-19. To expand the testing capacity, the government was compelled to recruit laboratory staff with limited experience and technical expertise, especially in molecular assays, to process specimens, interpret results, troubleshoot. As a result, the risk of diagnostic errors, such as cross-contamination, increased, potentially undermining the efficacy of public health policies, public health response, surveillance programs, and restrictive measures aimed toward containing the outbreak. In this piece, we discuss the different sources of cross-contamination in the COVID-19 RT-PCR laboratories and proffer practical preventive measures to avoid them.

关 键 词：SARS-CoV-2 RT-PCR Cross-Contaminations Quality Control COVID-19 False Positive 

分 类 号：R73[医药卫生—肿瘤]