Association between Serum Glutathione Peroxidases and Superoxide Dismutases mRNA Level with Coronary Artery Disease  

作　　者：Ali Reza Abaspour Mohammad Taghikhani Mohamad Reza Parizade Mohsen Moohebati Fahime Ghafoori Mehraneh Mehramiz Maryam Tayefi Amir Avan Marzeye Ghalandari Gordon A. A. Ferns Majid Ghayour-Mobarhan 

机构地区：[1]Department of Molecular Science, Faculty of Medicine, North Khorasan University of Medical Science, Bojnurd, Iran [2]Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran [3]Department of Biochemistry and Nutrition, Faculty of Medicine, Mashhad University of Medical Science (MUMS), Mashhad, Iran [4]Cardiovascular Research Center, Avicenna Research Institute, Mashhad University of Medical Science (MUMS), Mashhad, Iran [5]Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran [6]Department of Biochemistry, Faculty of Basic Science, Payame Noor University, Mashhad, Iran [7]Division of Medical Education, Brighton & Sussex Medical School, Brighton, UK

出　　处：《Health》2017年第2期252-260,共9页健康（英文）

摘　　要：Background: Oxidative stress plays a crucial role in the pathogenesis and progression of many diseases, including cardiovascular disease (CVD) and diabetes mellitus. Oxidative stress results from an imbalance between free radical formation and the protective antioxidant mechanisms. The latter mechanisms include superoxide dismutases (SODs) and glutathione peroxidases (GPx) that scavenge excessive ROS and protect cells against excess ROS production. The aim of current study was to determine the serum levels of SOD and serum GPx mRNA as well as the serum prooxidant-antioxidant balance in CVD patients. Method: A total of 103 subjects were recruited, with ≥50% stenosis (Angio+) or –). The expression levels of SOD and GPx in serum were measured using real time PCR. Biochemical-analyses (e.g., triglycerides;high-density lipo-protein cholesterol;low-density lipoprotein cholesterol;fasting-blood-glucose) were determined in all the subjects. Associations of SOD and GPx levels with biochemical and anthropometric characteristics were assessed together with evaluation of the serum pro-oxidant-antioxidant balance (PAB). Results: CVD subjects had a significantly higher level of fasting blood glucose (FBG), TC, LDL-C, TG and hs-CRP levels, as compared to control subjects. The level of serum PAB was significantly higher in the CVD group, 117.92 ± 35.51 and 110.65 ± 27.65 μg/dl in the angio– and angio+ groups, respectively compared to the control group (54.26 + 23.25). Additionally we observed that the SOD-3 level was higher in angio+ group versus control subjects. Conclusion: We have found that patients with CVD had a significantly higher prooxidant-antioxidant and SOD-3 levels. Further studies in larger multi-center setting are warranted to explore the value of emerging biomarker in CVD patients.Background: Oxidative stress plays a crucial role in the pathogenesis and progression of many diseases, including cardiovascular disease (CVD) and diabetes mellitus. Oxidative stress results from an imbalance between free radical formation and the protective antioxidant mechanisms. The latter mechanisms include superoxide dismutases (SODs) and glutathione peroxidases (GPx) that scavenge excessive ROS and protect cells against excess ROS production. The aim of current study was to determine the serum levels of SOD and serum GPx mRNA as well as the serum prooxidant-antioxidant balance in CVD patients. Method: A total of 103 subjects were recruited, with ≥50% stenosis (Angio+) or –). The expression levels of SOD and GPx in serum were measured using real time PCR. Biochemical-analyses (e.g., triglycerides;high-density lipo-protein cholesterol;low-density lipoprotein cholesterol;fasting-blood-glucose) were determined in all the subjects. Associations of SOD and GPx levels with biochemical and anthropometric characteristics were assessed together with evaluation of the serum pro-oxidant-antioxidant balance (PAB). Results: CVD subjects had a significantly higher level of fasting blood glucose (FBG), TC, LDL-C, TG and hs-CRP levels, as compared to control subjects. The level of serum PAB was significantly higher in the CVD group, 117.92 ± 35.51 and 110.65 ± 27.65 μg/dl in the angio– and angio+ groups, respectively compared to the control group (54.26 + 23.25). Additionally we observed that the SOD-3 level was higher in angio+ group versus control subjects. Conclusion: We have found that patients with CVD had a significantly higher prooxidant-antioxidant and SOD-3 levels. Further studies in larger multi-center setting are warranted to explore the value of emerging biomarker in CVD patients.

关 键 词：CORONARY ARTERY Disease GLUTATHIONE PEROXIDASES Superoxide Dismutases Real Time PCR ANGIOGRAPHY 

分 类 号：R5[医药卫生—内科学]