Resistance of Klebsiella to Imipenem by Production of Carbapenemase Gene bla_IMP at Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso  

作　　者：Blandine Ouédraogo Abdoul Karim Ouattara Amana Mètuor Dabiré Rahimatou Yasmine Wendkuni Tiemtoré Serge Sougé Jacques Simporé Blandine Ouédraogo;Abdoul Karim Ouattara;Amana Mètuor Dabiré;Rahimatou Yasmine Wendkuni Tiemtoré;Serge Sougé;Jacques Simporé(Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE), UFR-SVT, Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso;Université Norbert Zongo, Centre Universitaire de Manga, Koudougou, Burkina Faso;Département de Biochimie-Microbiologie, Université de Dédougou, Dédougou, Burkina Faso;Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso)

机构地区：[1]Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE), UFR-SVT, Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso [2]Université Norbert Zongo, Centre Universitaire de Manga, Koudougou, Burkina Faso [3]Département de Biochimie-Microbiologie, Université de Dédougou, Dédougou, Burkina Faso [4]Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso

出　　处：《International Journal of Clinical Medicine》2023年第8期347-356,共9页临床医学国际期刊（英文）

摘　　要：Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β-lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene bla_IMP in the genus Klebsiella at the Charles De Gaulle Pediatric University Hospital (CHUP-CDG) of Ouagadougou, Burkina Faso. Methods: The study involved 17 bacterial strains responsible for human infection and isolated from various biological samples during the period from 2009 to 2013. The strains were tested for antimicrobial susceptibility to cefotaxime, ceftazidime and imipenem using the Mueller-Hinton agar diffusion method. The carbapenemases resistance genes were detected by conventional PCR using specific primers at the molecular biology laboratory of CERBA/LABIOGENE, Ouagadougou, Burkina Faso. Results: The antibiotic susceptibility test showed high resistance of the 17 Klebsiella isolates tested to cephalosporins. A high cefotaxime-resistance rate (82.35%) and ceftazidime-resistance rate (88.23%) was found among the strains tested against 11.76% resistance rate for imipenem. Analysis of PCR products by gel electrophoresis revealed 4 strains (23.53%) with bla_IMP-type gene. Conclusion: Klebsiella is a well-known bacterium in clinical practice. The present study demonstrated the bla_IMP-type gene in cephalosporin-resistant strains of Klebsiella at CHUP-CDG. More effective monitoring and treatment solutions are needed to prevent the spread of these resistant strains.Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β-lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene bla_IMP in the genus Klebsiella at the Charles De Gaulle Pediatric University Hospital (CHUP-CDG) of Ouagadougou, Burkina Faso. Methods: The study involved 17 bacterial strains responsible for human infection and isolated from various biological samples during the period from 2009 to 2013. The strains were tested for antimicrobial susceptibility to cefotaxime, ceftazidime and imipenem using the Mueller-Hinton agar diffusion method. The carbapenemases resistance genes were detected by conventional PCR using specific primers at the molecular biology laboratory of CERBA/LABIOGENE, Ouagadougou, Burkina Faso. Results: The antibiotic susceptibility test showed high resistance of the 17 Klebsiella isolates tested to cephalosporins. A high cefotaxime-resistance rate (82.35%) and ceftazidime-resistance rate (88.23%) was found among the strains tested against 11.76% resistance rate for imipenem. Analysis of PCR products by gel electrophoresis revealed 4 strains (23.53%) with bla_IMP-type gene. Conclusion: Klebsiella is a well-known bacterium in clinical practice. The present study demonstrated the bla_IMP-type gene in cephalosporin-resistant strains of Klebsiella at CHUP-CDG. More effective monitoring and treatment solutions are needed to prevent the spread of these resistant strains.

关 键 词：KLEBSIELLA RESISTANCE bla_IMPGenes Β-LACTAM Burkina Faso 

分 类 号：R44[医药卫生—诊断学]