Evolution of Erythromycin Resistance among Streptococcus pneumoniae Isolates in Malaysia from 2005 and 2010  

作　　者：Revathy Arushothy Norazah Ahmad Rohani Md Yassin Revathy Arushothy;Norazah Ahmad;Rohani Md Yassin(Bacteriology Unit, Infectious Disease Research Center, Institute for Medical Research, Kuala Lumpur, Malaysia;Special Diagnostic Research Center, Institute for Medical Research, Kuala Lumpur, Malaysia)

机构地区：[1]Bacteriology Unit, Infectious Disease Research Center, Institute for Medical Research, Kuala Lumpur, Malaysia [2]Special Diagnostic Research Center, Institute for Medical Research, Kuala Lumpur, Malaysia

出　　处：《Journal of Biosciences and Medicines》2016年第5期116-122,共7页生物科学与医学（英文）

摘　　要：Objectives: This study focuses on the antibiotic susceptibility pattern and distribution of the ermB and mefA virulence genes among the Streptococcus pneumoniae due to an increase in erythromycin resistance in S. pneumoniae worldwide. Methodology: We investigated 255 clinical isolates collected from 2005-2010 to determine the serotype distribution and resistance to erythromycin in comparison to penicillin, clindamycin, clarithromycin, azithromycin, and trimethoprim sulfamethoxazole. Multiplex PCR was carried out to detect erythromycin resistance genes (ermB and mefA). Results: There were 146 (57.3%) isolates resistant to erythromycin. MIC₉₀ for erythromycin is at >256 mg/L and MIC₅₀ is at 16 mg/L. The ermB gene was detected in 25.3% of the erythromycin-resistant isolates and mefA gene was detected in 50.7% of the isolates. The four most common serotypes encountered are 19F, 19A, 23F and 14. The serotype distribution among the erythromycin resistant isolates was 19F (42.0%) followed by serotype 19A (11.3%), serotype 23F (9.2%) and serogroup 14 (7.0%). Conclusion: In conclusion, there is a significant rise in erythromycin resistance among the Malaysian pneumococcal isolates. The emergence of serotype 19A together with increasing prevalence of resistance to macrolide warrants for a more extensive surveillance study.Objectives: This study focuses on the antibiotic susceptibility pattern and distribution of the ermB and mefA virulence genes among the Streptococcus pneumoniae due to an increase in erythromycin resistance in S. pneumoniae worldwide. Methodology: We investigated 255 clinical isolates collected from 2005-2010 to determine the serotype distribution and resistance to erythromycin in comparison to penicillin, clindamycin, clarithromycin, azithromycin, and trimethoprim sulfamethoxazole. Multiplex PCR was carried out to detect erythromycin resistance genes (ermB and mefA). Results: There were 146 (57.3%) isolates resistant to erythromycin. MIC₉₀ for erythromycin is at >256 mg/L and MIC₅₀ is at 16 mg/L. The ermB gene was detected in 25.3% of the erythromycin-resistant isolates and mefA gene was detected in 50.7% of the isolates. The four most common serotypes encountered are 19F, 19A, 23F and 14. The serotype distribution among the erythromycin resistant isolates was 19F (42.0%) followed by serotype 19A (11.3%), serotype 23F (9.2%) and serogroup 14 (7.0%). Conclusion: In conclusion, there is a significant rise in erythromycin resistance among the Malaysian pneumococcal isolates. The emergence of serotype 19A together with increasing prevalence of resistance to macrolide warrants for a more extensive surveillance study.

关 键 词：Streptococcus pneumoniae PNEUMOCOCCAL Erythromycin Resistance SEROTYPE mefA ermB 

分 类 号：R96[医药卫生—药理学]