Bacteremia in Subjects with Sickle Cell Disease: High Rate of Gram-Negative Isolates in the West African Context!  

作　　者：Papa Silman Diawara Sokhna Moumy Daffe Mamadou Wague Gueye Khadija Fall Moustapha Diop Aminata Diop Nakoulima Tagoutie Niang Mbene Fall Alice Ingabire Mor Ngom Maguette Ndoye Nata Dieng Bécaye Fall Macoura Gadji Meissa Ndew Seye Pape Samba Ba Yankhoba Diop Papa Silman Diawara;Sokhna Moumy Daffe;Mamadou Wague Gueye;Khadija Fall;Moustapha Diop;Aminata Diop Nakoulima;Tagoutie Niang;Mbene Fall;Alice Ingabire;Mor Ngom;Maguette Ndoye;Nata Dieng;Bécaye Fall;Macoura Gadji;Meissa Ndew Seye;Pape Samba Ba;Yankhoba Diop(Federation of Laboratories, Dakar Principal Hospital, Dakar, Senegal;Pediatrics Department, Dakar Principal Hospital, Dakar, Senegal;Infectious Diseases Department, Dakar Principal Hospital, Dakar, Senegal;Hematology Department, Dakar Principal Hospital, Dakar, Senegal;Department of Biological Hematology & Oncology-Hematology (HBOH), National Blood Transfusion Center (CNTS)/FMPOS-UCAD, Dakar, Senegal)

机构地区：[1]Federation of Laboratories, Dakar Principal Hospital, Dakar, Senegal [2]Pediatrics Department, Dakar Principal Hospital, Dakar, Senegal [3]Infectious Diseases Department, Dakar Principal Hospital, Dakar, Senegal [4]Hematology Department, Dakar Principal Hospital, Dakar, Senegal [5]Department of Biological Hematology & Oncology-Hematology (HBOH), National Blood Transfusion Center (CNTS)/FMPOS-UCAD, Dakar, Senegal

出　　处：《Journal of Biosciences and Medicines》2023年第2期15-29,共15页生物科学与医学（英文）

摘　　要：Background: Sickle cell disease is one of the most common monogenic diseases in the world, affecting approximately 70 million people, 80% in sub-Saharan Africa and 1 in 10 in Senegal. Sickle cell anemia causes functional asplenia (associated with repeated thrombosis of splenic vessels), resulting in increased susceptibility to infection. However, several studies have reported differences in the spectrum of bacterial infections in malaria-endemic areas. Therefore, we proposed to conduct a study to determine the rate of positive blood cultures and the bacteriological spectrum in sickle cell patients. Materials and Method: This is a descriptive cross-sectional study of blood culture samples from patients who received a request for hemoglobin electrophoresis as part of their treatment at the Principal’s hospital in Dakar. The study took place from January 2008 to December 2021. For each patient, we collect demographic information, including age, gender, and the service from which the analysis request originated. Data were collected in the laboratory’s computer system and entered into Microsoft Excel (2007). Statistical analyzes were performed using Epi-Info 7 software. Results: Our study included 1419 patients. The most common types of hemoglobin profiles were: normal profile (n = 1025), AS profile (n = 283), SS profile (n = 104), SC profile (n = 7). This corresponds to the proportions of 72%, 20%, 0.5% and 7.5% for the profiles Normal, AS, SC and SS. The male proportion was 61.1%, 61.5%, 57.1, respectively %, 55.8% for Normal, SA, SC, and SS profiles. A total of 19,090 individual blood culture bottles were collected from 1419 impatient patients as follows: Normal profile (n = 18,042 bottles), AS profile (n = 677 culture bottles), SS profile (n = 362 bottles). The majority of blood culture orders come from pediatric services, accounting for 70% of the total number of orders. Of 19,090 vials examined in this current study, 19.6% developed a positive blood culture. Overall, the most commonly isolated bacteria were Background: Sickle cell disease is one of the most common monogenic diseases in the world, affecting approximately 70 million people, 80% in sub-Saharan Africa and 1 in 10 in Senegal. Sickle cell anemia causes functional asplenia (associated with repeated thrombosis of splenic vessels), resulting in increased susceptibility to infection. However, several studies have reported differences in the spectrum of bacterial infections in malaria-endemic areas. Therefore, we proposed to conduct a study to determine the rate of positive blood cultures and the bacteriological spectrum in sickle cell patients. Materials and Method: This is a descriptive cross-sectional study of blood culture samples from patients who received a request for hemoglobin electrophoresis as part of their treatment at the Principal’s hospital in Dakar. The study took place from January 2008 to December 2021. For each patient, we collect demographic information, including age, gender, and the service from which the analysis request originated. Data were collected in the laboratory’s computer system and entered into Microsoft Excel (2007). Statistical analyzes were performed using Epi-Info 7 software. Results: Our study included 1419 patients. The most common types of hemoglobin profiles were: normal profile (n = 1025), AS profile (n = 283), SS profile (n = 104), SC profile (n = 7). This corresponds to the proportions of 72%, 20%, 0.5% and 7.5% for the profiles Normal, AS, SC and SS. The male proportion was 61.1%, 61.5%, 57.1, respectively %, 55.8% for Normal, SA, SC, and SS profiles. A total of 19,090 individual blood culture bottles were collected from 1419 impatient patients as follows: Normal profile (n = 18,042 bottles), AS profile (n = 677 culture bottles), SS profile (n = 362 bottles). The majority of blood culture orders come from pediatric services, accounting for 70% of the total number of orders. Of 19,090 vials examined in this current study, 19.6% developed a positive blood culture. Overall, the most commonly isolated bacteria were

关 键 词：Sickle Cell Disease Senegal BACTEREMIA Blood Culture Infection 

分 类 号：R73[医药卫生—肿瘤]