A Preliminary Mechanism Study on the Treatment of Rabbit Bone Defect Bone Nonunion with Xuduan Jiegu Decoction  

作　　者：Zengping Lin Kanghua Zheng Darong Nie Huoquan Ye Hongjie Zhang Linfeng Tang Zhong Dong Zengping Lin;Kanghua Zheng;Darong Nie;Huoquan Ye;Hongjie Zhang;Linfeng Tang;Zhong Dong(Department of Orthopaedics, Fujian Provincial 2nd Peoples Hospital, Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, China;Fujian Provincial 2nd Peoples Hospital, Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, China)

机构地区：[1]Department of Orthopaedics, Fujian Provincial 2nd Peoples Hospital, Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, China [2]Fujian Provincial 2nd Peoples Hospital, Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, China

出　　处：《Journal of Biosciences and Medicines》2024年第10期297-303,共7页生物科学与医学（英文）

摘　　要：Objective: To investigate the curative effect and mechanism of Xuduan Jiegu Decoction in the treatment of rabbit bone defect bone nonunion. Methods: The experiment was randomly divided into three groups;1) The control group that was given neither the modeling nor the treatment;2) The model group that was not given the treatment after the operation to model a bone defect;3) The treatment group that was given intermittent bone decoction after modeling (i.e., the operation), the dose was 105 g/piece, twice a day, for 4 consecutive weeks. For the three groups, Anterolateral X-rays of the left forearm were taken 14 days after the surgery to observe the bone nonunion healing with pretherapy and post-treatment. The expression levels of TGF-β, BMP-2 and VEGF in the blood of each group were measured by ELISA at 4 weeks after treatment. The peri-fracture histopathological changes between each group of pretherapy and post-treatment were evaluated by using tissue sections. Results: Compared with the control group, there was no obvious healing in the model group on the 14th day after the operation. Compared with the model group, the treatment group was treated with Xuduan Jiegu Decoction, and there was a trend of healing and callus formation on the 14th day. HE staining showed that the cells in the control group were closely arranged without any pathological changes. In the model group, the tissues around the fracture end were arranged loosely, and the cells were vacuolated and infiltrated by inflammatory cells. Compared with the model group, the peripheral cell arrangement was better and the peripheral lesions were reduced in the treatment group. The content of TGF-β, BMP-2 and VEGF in blood detected by ELISA was significantly higher in the treatment group than in the control group and the model group, with statistical significance. Conclusion: Xuduan Jiegu Decoction can promote callus formation and accelerate fracture healing in rabbit radius bone defect, and the mechanism of promoting fracture healing is related to the iObjective: To investigate the curative effect and mechanism of Xuduan Jiegu Decoction in the treatment of rabbit bone defect bone nonunion. Methods: The experiment was randomly divided into three groups;1) The control group that was given neither the modeling nor the treatment;2) The model group that was not given the treatment after the operation to model a bone defect;3) The treatment group that was given intermittent bone decoction after modeling (i.e., the operation), the dose was 105 g/piece, twice a day, for 4 consecutive weeks. For the three groups, Anterolateral X-rays of the left forearm were taken 14 days after the surgery to observe the bone nonunion healing with pretherapy and post-treatment. The expression levels of TGF-β, BMP-2 and VEGF in the blood of each group were measured by ELISA at 4 weeks after treatment. The peri-fracture histopathological changes between each group of pretherapy and post-treatment were evaluated by using tissue sections. Results: Compared with the control group, there was no obvious healing in the model group on the 14th day after the operation. Compared with the model group, the treatment group was treated with Xuduan Jiegu Decoction, and there was a trend of healing and callus formation on the 14th day. HE staining showed that the cells in the control group were closely arranged without any pathological changes. In the model group, the tissues around the fracture end were arranged loosely, and the cells were vacuolated and infiltrated by inflammatory cells. Compared with the model group, the peripheral cell arrangement was better and the peripheral lesions were reduced in the treatment group. The content of TGF-β, BMP-2 and VEGF in blood detected by ELISA was significantly higher in the treatment group than in the control group and the model group, with statistical significance. Conclusion: Xuduan Jiegu Decoction can promote callus formation and accelerate fracture healing in rabbit radius bone defect, and the mechanism of promoting fracture healing is related to the i

关 键 词：Xuduan Jiegu Decoction Bone Nonunion TGF-β BMP-2 VEGF 

分 类 号：R73[医药卫生—肿瘤]