Spread of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Clinical Isolates from Patients in Bobo-Dioulasso, Burkina Faso  

作　　者：Kobo Gnada Michel Kiréopori Gomgnimbou Armel Moumouni Sanou Louis Robert W. Belem Azouman Da Arnaud Quetin Sanou Do Malick Soufiane Sanou Kobo Gnada;Michel Kiréopori Gomgnimbou;Armel Moumouni Sanou;Louis Robert W. Belem;Azouman Da;Arnaud Quetin Sanou;Do Malick Soufiane Sanou(Equipe Biologie Molculaire et Biotechnologies, Laboratoire de Recherche, Centre MURAZ, Institut National de Sant Publique, Bobo-Dioulasso, Burkina Faso;Centre dExcellence Africain en Innovations Biotechnologiques pour lElimination des Maladies Transmission Vectorielle (CEA/ITECH-MTV), Universit Nazi BONI, Bobo-Dioulasso, Burkina Faso;Institut Suprieur des Sciences de la Sant (IN.S.SA), Universit Nazi BONI, Bobo-Dioulasso, Burkina Faso;Laboratoire de Recherche sur les Maladies Infectieuses et Parasitaire (LR-MIP), Institut de Recherche en Science de la Sante, Bobo-Dioulasso, Burkina Faso;Equipe Bactriologie-Mycobactriologie, Laboratoire de Recherche, Centre MURAZ, Institut National de Sant Publique, Bobo-Dioulasso, Burkina Faso)

机构地区：[1]Equipe Biologie Molculaire et Biotechnologies, Laboratoire de Recherche, Centre MURAZ, Institut National de Sant Publique, Bobo-Dioulasso, Burkina Faso [2]Centre dExcellence Africain en Innovations Biotechnologiques pour lElimination des Maladies Transmission Vectorielle (CEA/ITECH-MTV), Universit Nazi BONI, Bobo-Dioulasso, Burkina Faso [3]Institut Suprieur des Sciences de la Sant (IN.S.SA), Universit Nazi BONI, Bobo-Dioulasso, Burkina Faso [4]Laboratoire de Recherche sur les Maladies Infectieuses et Parasitaire (LR-MIP), Institut de Recherche en Science de la Sante, Bobo-Dioulasso, Burkina Faso [5]Equipe Bactriologie-Mycobactriologie, Laboratoire de Recherche, Centre MURAZ, Institut National de Sant Publique, Bobo-Dioulasso, Burkina Faso

出　　处：《Journal of Biosciences and Medicines》2025年第1期394-405,共12页生物科学与医学(英文)

摘　　要：Antimicrobial resistance (AMR) is one of the top 10 threats to global health and it is estimated around 10 millions of deaths per year are associated with AMR until 2050. Burkina Faso is also facing the emergence and spread of AMR of several bacteria resistant strains such as those of public health concerns under surveillance Enterobacteriaceae. The aim of this study was to assess the prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) clinical isolates from patients attending the bacteriology laboratory of the Centre MURAZ in Bobo-Dioulasso, Burkina Faso. Clinical isolates from urine, pus, stool, and semen were collected from April to June 2017. Identification and antibiotic susceptibility testing were performed using the VITEK 2 compact automated system according to EUCAST version 2015 recommendations. ESBL detection was then performed on the Muller-Hinton medium using the combined disc method. One hundred (100) strains of Enterobacteriaceae were isolated from 100 patients, including 52% of ESBLS. Escherichia coli (E. coli) was the most commonly isolated ESBL [(84.62, 44/72) ESBL], followed by Klebsiella spp. [(40%, 06/15) ESBL], then Enterobacter spp. [(40%, 2/5) ESBL]. Risk factor analysis revealed that ESBL-PE infection was frequently found in pus samples (P = 0.042;[OR] = 3.16;95% [CI] = 1.04 - 9.61) and that E. coli was the strain most likely to harbour ESBL (P = 0.008;[OR] = 3.60;95% [CI] = 1.40 - 9.31). This study reports a high prevalence of ESBL-PE associated with strong resistance to quinolones and cotrimoxazole (over 80%), which calls for increased surveillance of these superbugs, the adoption of a rational antibiotic prescription policy, and rigorous hygiene measures to prevent the spread of these multi-resistant bacteria.Antimicrobial resistance (AMR) is one of the top 10 threats to global health and it is estimated around 10 millions of deaths per year are associated with AMR until 2050. Burkina Faso is also facing the emergence and spread of AMR of several bacteria resistant strains such as those of public health concerns under surveillance Enterobacteriaceae. The aim of this study was to assess the prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) clinical isolates from patients attending the bacteriology laboratory of the Centre MURAZ in Bobo-Dioulasso, Burkina Faso. Clinical isolates from urine, pus, stool, and semen were collected from April to June 2017. Identification and antibiotic susceptibility testing were performed using the VITEK 2 compact automated system according to EUCAST version 2015 recommendations. ESBL detection was then performed on the Muller-Hinton medium using the combined disc method. One hundred (100) strains of Enterobacteriaceae were isolated from 100 patients, including 52% of ESBLS. Escherichia coli (E. coli) was the most commonly isolated ESBL [(84.62, 44/72) ESBL], followed by Klebsiella spp. [(40%, 06/15) ESBL], then Enterobacter spp. [(40%, 2/5) ESBL]. Risk factor analysis revealed that ESBL-PE infection was frequently found in pus samples (P = 0.042;[OR] = 3.16;95% [CI] = 1.04 - 9.61) and that E. coli was the strain most likely to harbour ESBL (P = 0.008;[OR] = 3.60;95% [CI] = 1.40 - 9.31). This study reports a high prevalence of ESBL-PE associated with strong resistance to quinolones and cotrimoxazole (over 80%), which calls for increased surveillance of these superbugs, the adoption of a rational antibiotic prescription policy, and rigorous hygiene measures to prevent the spread of these multi-resistant bacteria.

关 键 词：ENTEROBACTERIACEAE ESBL RESISTANCE ANTIBIOGRAM PHENOTYPES Burkina Faso 

分 类 号：R44[医药卫生—诊断学]