Porcine Placenta Extract Reduced Wrinkle Formation by Potentiating Epidermal Hydration  

作　　者：Akihiro Aioi Ryuta Muromoto Sho Mogami Megumi Nishikawa Shigeyuki Ogawa Tadashi Matsuda Akihiro Aioi;Ryuta Muromoto;Sho Mogami;Megumi Nishikawa;Shigeyuki Ogawa;Tadashi Matsuda(New Business Development Division, Septem-Soken, Osaka, Japan;Department of Immunology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan)

机构地区：[1]New Business Development Division, Septem-Soken, Osaka, Japan [2]Department of Immunology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan

出　　处：《Journal of Cosmetics, Dermatological Sciences and Applications》2021年第2期101-109,共9页化妆品、皮肤病及应用期刊（英文）

摘　　要：<strong>Background:</strong> Placenta extract is used as an alternative medicine especially in Asian countries as it is a rich reservoir of diverse bioactive molecules. The effects of placenta extract on skin conditions have been previously reported, however, the mechanism underlying for reduced wrinkle formation remains unclear. <strong>Objective:</strong> The primary objective of this study was to determine whether the continuous application of porcine placenta extract (PPE) alleviates wrinkle formation in humans and explore the underlying mechanism. <strong>Methods:</strong> Wrinkle formation, skin hydration, and skin elasticity were measured in 15 volunteers at weeks 0 and 6 after continuous application of a gel containing PPE. The production of type I collagen and hyaluronic acid from fibroblasts and keratinocytes, respectively, were measured using ELISA. Expression levels of ceramide synthase 3 (CERS3), filaggrin (FLG), transglutaminase 1 (TGM1), and kallikrein-7 (KLK7) were evaluated by quantitative real-time PCR. <strong>Results:</strong> The wrinkle index was significantly reduced to 72.1% after a 6-week of applying the PPE gel, along with a significant increase in skin hydration to 126.5%. Type I collagen production from fibroblasts was enhanced slightly but significantly following treatment with PPE. PPE accelerated the expression of CERS3 (1.85-fold), FLG (1.35-fold), TGM1 (1.76-fold), and KLK7 (1.62-fold). <strong>Conclusion:</strong> Treatment with PPE alleviates wrinkle formation and simultaneously enhances skin hydration, which is induced via the accelerated expression of moisturizing-related proteins. These findings suggest that PPE is effective for combating dryness-induced wrinkle formation.<strong>Background:</strong> Placenta extract is used as an alternative medicine especially in Asian countries as it is a rich reservoir of diverse bioactive molecules. The effects of placenta extract on skin conditions have been previously reported, however, the mechanism underlying for reduced wrinkle formation remains unclear. <strong>Objective:</strong> The primary objective of this study was to determine whether the continuous application of porcine placenta extract (PPE) alleviates wrinkle formation in humans and explore the underlying mechanism. <strong>Methods:</strong> Wrinkle formation, skin hydration, and skin elasticity were measured in 15 volunteers at weeks 0 and 6 after continuous application of a gel containing PPE. The production of type I collagen and hyaluronic acid from fibroblasts and keratinocytes, respectively, were measured using ELISA. Expression levels of ceramide synthase 3 (CERS3), filaggrin (FLG), transglutaminase 1 (TGM1), and kallikrein-7 (KLK7) were evaluated by quantitative real-time PCR. <strong>Results:</strong> The wrinkle index was significantly reduced to 72.1% after a 6-week of applying the PPE gel, along with a significant increase in skin hydration to 126.5%. Type I collagen production from fibroblasts was enhanced slightly but significantly following treatment with PPE. PPE accelerated the expression of CERS3 (1.85-fold), FLG (1.35-fold), TGM1 (1.76-fold), and KLK7 (1.62-fold). <strong>Conclusion:</strong> Treatment with PPE alleviates wrinkle formation and simultaneously enhances skin hydration, which is induced via the accelerated expression of moisturizing-related proteins. These findings suggest that PPE is effective for combating dryness-induced wrinkle formation.

关 键 词：Porcine Placenta Wrinkle Formation Moisturizing-Related Proteins Keratinocyte Differentiation 

分 类 号：R73[医药卫生—肿瘤]