QBC ParaLens^(TM) LED fluorescent microscope attachment with QBC F.A.S.T.^(TM) AFB staining system  

作　　者：Kouassi N’Guessan Brian Joseph Hnatkovich N’Guetta Aka Armand Achy-Brou Bakary Coulibaly Jean Marc Assande Philomene Mohoudi Mireille Dosso 

机构地区：[1]Departement de Bacteriologie-Virologie,Institut Pasteur de Cote d’Ivoire,Abidjan,Cote d’Ivoire [2]QBC Diagnostics,Inc.,Port Matilda,USA [3]Departement d’Epidemiologie,Institut Pasteur de Cote d’Ivoire,Abidjan,Cote d’Ivoire

出　　处：《Journal of Tuberculosis Research》2013年第3期40-43,共4页结核病研究（英文）

基　　金：Mr. Alain Noudofinin

摘　　要：This study was designed to evaluate the QBC ParaLensTM LED fluorescent microscope attachment and the QBC F.A.S.T.TM AFB staining system for the detection of Acid Fast Bacilli in pathological samples in Abidjan, Cote d’Ivoire. A total of 50 patients were tested using direct smear specimens with both Ziehl-Neelsen (ZN) light microscopy and LED fluorescence microscopy with QBC F.A.S.T. AFB stain. The samples were also cultured and tested using an immunochromatograpic test for detection of antigen MPT 64 and the results were compared to direct examination. ZN light microscopy detected 20 positive cases and LED fluorescent microscopy with QBC F.A.S.T. AFB stain detected 21. The sensitivity and specificity of ZN light microscopy was determined to be 84.2% and 87.1% respectively. The sensitivity and specificity of LED fluorescent microscopy with QBC F.A.S.T. AFB stain was determined to be 94.7% and 90.3% respectively. Compared to ZN light microscopy, LED fluorescent microscopy with QBC F.A.S.T. AFB stain increased the sensitivity of direct examination without concentration by 10.5%.This study was designed to evaluate the QBC ParaLensTM LED fluorescent microscope attachment and the QBC F.A.S.T.TM AFB staining system for the detection of Acid Fast Bacilli in pathological samples in Abidjan, Cote d’Ivoire. A total of 50 patients were tested using direct smear specimens with both Ziehl-Neelsen (ZN) light microscopy and LED fluorescence microscopy with QBC F.A.S.T. AFB stain. The samples were also cultured and tested using an immunochromatograpic test for detection of antigen MPT 64 and the results were compared to direct examination. ZN light microscopy detected 20 positive cases and LED fluorescent microscopy with QBC F.A.S.T. AFB stain detected 21. The sensitivity and specificity of ZN light microscopy was determined to be 84.2% and 87.1% respectively. The sensitivity and specificity of LED fluorescent microscopy with QBC F.A.S.T. AFB stain was determined to be 94.7% and 90.3% respectively. Compared to ZN light microscopy, LED fluorescent microscopy with QBC F.A.S.T. AFB stain increased the sensitivity of direct examination without concentration by 10.5%.

关 键 词：TUBERCULOSIS AFB Ziehl-Neelsen LED Fluorescence QBC ParaLens QBC F.A.S.T. 

分 类 号：R73[医药卫生—肿瘤]