Anti-Tubercular Activities and Molecular Characterization of Salivary Extract of Leech (<i>Hirudo medicinalis</i>) against <i>Mycobacterium tuberculosis</i>  

作　　者：Praise Olufunmilola Ojo Hausatu Babayi Israel Kayode Olayemi Oladosun Oluwale Peter Labake Ajoke Fadipe Elisha Baba Kasim Izebe 

机构地区：[1]Department of Microbiology, Federal University of Technology, Minna, Nigeria [2]Department of Biological Sciences, Federal University of Technology, Minna, Nigeria [3]Department of Microbiology, National Institute for Pharmaceutical Research and Development, Idu-Abuja, Nigeria [4]Department of Chemistry, Federal University of Technology, Minna, Nigeria [5]Clinic, National Institute for Pharmaceutical Research and Development, Idu-Abuja, Nigeria

出　　处：《Journal of Tuberculosis Research》2018年第1期1-9,共9页结核病研究（英文）

摘　　要：Leech salivary extract (LSE) was screened for the anti-tubercular activity against Mycobacterium tuberculosis using microbroth dilution method. The bioactive components in LSE were determined using Shimadzu GC-MS QP2010 SE. The LSE exhibited bactericidal effects on M. tuberculosis at 50% (v/v). The result of the GC-MS analysis of LSE revealed seventeen (17) bioactive components. The major fatty acid components were oleic acid (33.9%) and palmitic acid also known as hexadecanoic acid (22.6%). Other bioactive components detected in appreciable amounts in LSE include, 4-bromobutyric acid (16.86%), 6,17-Octadiene-1-ol acetate (9.78%) and octahydro-1,4,9,9- tetramethyl (5.21%). The anti-tubercular activity of the LSE was attributed to these bioactive components. The results obtained suggest that leech salivary extract could be used in treating tuberculosis.Leech salivary extract (LSE) was screened for the anti-tubercular activity against Mycobacterium tuberculosis using microbroth dilution method. The bioactive components in LSE were determined using Shimadzu GC-MS QP2010 SE. The LSE exhibited bactericidal effects on M. tuberculosis at 50% (v/v). The result of the GC-MS analysis of LSE revealed seventeen (17) bioactive components. The major fatty acid components were oleic acid (33.9%) and palmitic acid also known as hexadecanoic acid (22.6%). Other bioactive components detected in appreciable amounts in LSE include, 4-bromobutyric acid (16.86%), 6,17-Octadiene-1-ol acetate (9.78%) and octahydro-1,4,9,9- tetramethyl (5.21%). The anti-tubercular activity of the LSE was attributed to these bioactive components. The results obtained suggest that leech salivary extract could be used in treating tuberculosis.

关 键 词：GC-MS MYCOBACTERIUM tuberculosis LEECH SALIVARY EXTRACT 

分 类 号：R73[医药卫生—肿瘤]