Molecular Characterization of Gastrointestinal Stromal Tumors (Gist) and Contribution of Immunohistochemistry in Congolese from Kinshasa  

作　　者：Rive Lukuaku Jérémie Okumadi Fabrice Bokambandja Andy Mbangama Vincent Mbatu Georges Mvumbi Jean-Louis Mumbere David Azako Serge Pezo Dieudonné Mvumbi Rive Lukuaku;Jérémie Okumadi;Fabrice Bokambandja;Andy Mbangama;Vincent Mbatu;Georges Mvumbi;Jean-Louis Mumbere;David Azako;Serge Pezo;Dieudonné Mvumbi(Faculty of Medicine, Bel Campus Technological University, Kinshasa, DR Congo;Department of Anatomy and Pathological Cytology, University Clinics of Kinshasa, Kinshasa, DR Congo;Department of Gyneco-Obstetrics, University Clinics of Kinshasa, Kinshasa, DR Congo;Molecular Biology Department, Faculty of Medicine, University of Kinshasa, Kinshasa, DR Congo;Institute of Medical Immunology, Free University of Brussels, Ixelles, Belgium)

机构地区：[1]Faculty of Medicine, Bel Campus Technological University, Kinshasa, DR Congo [2]Department of Anatomy and Pathological Cytology, University Clinics of Kinshasa, Kinshasa, DR Congo [3]Department of Gyneco-Obstetrics, University Clinics of Kinshasa, Kinshasa, DR Congo [4]Molecular Biology Department, Faculty of Medicine, University of Kinshasa, Kinshasa, DR Congo [5]Institute of Medical Immunology, Free University of Brussels, Ixelles, Belgium

出　　处：《Open Journal of Gastroenterology》2024年第4期109-116,共8页肠胃病学期刊（英文）

摘　　要：Introduction: The differentiation of digestive tumors very often requires the use of techniques currently not widely in use in the Democratic Republic of Congo (DRC), such as immunohistochemistry. This is perfectly verified for GISTs whose precise, or at least highly certain, diagnosis can only be made using immunohistochemical markers. This underuse of these techniques due to lack of equipment and human skills explains the limited epidemiological data available to date, thus leading to untargeted and too often late treatment of patients. Research question: What contribution can immunohistochemical markers make to the diagnosis of digestive tract tumours? Objective: Discuss the contribution of immunohistochemical markers in the diagnosis of GIST and provide basic data on the epidemiology of these nosological entities in Kinshasa. Methodology: This was a retrospective study carried out at the LEBOMA private anatomy and pathological cytology centre. The main inclusion criterion was any digestive tract block or slide whose diagnosis of GIST had been requalified after review by at least 2 pathologists. An immuhistochemical study was performed using an automated technique (with a Ventana XT machine) using a panel of antibodies: CD-117 and DOG-1 which are listed in the literature as strongly correlated with the occurrence of GIST, all slides were made at Hj Hospital using an OLYMPUS BX41 co-observation microscope. Results: Of 601 cases of digestive tumors recorded during the concerned period, 32 (5.32%) concerned GIST. This prevalence was confirmed by our immunohistochemical results where the expression of CD117 and that of DOG-1 were positive in 90.6% and 100% of cases which prevalence is high compared with the worldwide prevalence according to the literature, respectively. The distribution of the patients concerned was made with a sex ratio of 1.6 women/men with a median age of 53 years. Most cases (81%) had a gastric location and were fusiform GISTs. Conclusion: Gastrointestinal stromal tumours, although rare and unIntroduction: The differentiation of digestive tumors very often requires the use of techniques currently not widely in use in the Democratic Republic of Congo (DRC), such as immunohistochemistry. This is perfectly verified for GISTs whose precise, or at least highly certain, diagnosis can only be made using immunohistochemical markers. This underuse of these techniques due to lack of equipment and human skills explains the limited epidemiological data available to date, thus leading to untargeted and too often late treatment of patients. Research question: What contribution can immunohistochemical markers make to the diagnosis of digestive tract tumours? Objective: Discuss the contribution of immunohistochemical markers in the diagnosis of GIST and provide basic data on the epidemiology of these nosological entities in Kinshasa. Methodology: This was a retrospective study carried out at the LEBOMA private anatomy and pathological cytology centre. The main inclusion criterion was any digestive tract block or slide whose diagnosis of GIST had been requalified after review by at least 2 pathologists. An immuhistochemical study was performed using an automated technique (with a Ventana XT machine) using a panel of antibodies: CD-117 and DOG-1 which are listed in the literature as strongly correlated with the occurrence of GIST, all slides were made at Hj Hospital using an OLYMPUS BX41 co-observation microscope. Results: Of 601 cases of digestive tumors recorded during the concerned period, 32 (5.32%) concerned GIST. This prevalence was confirmed by our immunohistochemical results where the expression of CD117 and that of DOG-1 were positive in 90.6% and 100% of cases which prevalence is high compared with the worldwide prevalence according to the literature, respectively. The distribution of the patients concerned was made with a sex ratio of 1.6 women/men with a median age of 53 years. Most cases (81%) had a gastric location and were fusiform GISTs. Conclusion: Gastrointestinal stromal tumours, although rare and un

关 键 词：Digestive Tumors GIST IMMUNOHISTOCHEMISTRY CD117 DOG-1 

分 类 号：R73[医药卫生—肿瘤]