CD62L Percentage in Peripheral T Cells of Kidney Transplant Recipients Children  

作　　者：Alaa Rashad Fatina I. Fadel Doaa M. Salah Eman A. Elghoroury Neemat M. A. Kassem Eman Mahmoud Mervat Ismail Manal F. Elshamaa Alaa Rashad;Fatina I. Fadel;Doaa M. Salah;Eman A. Elghoroury;Neemat M. A. Kassem;Eman Mahmoud;Mervat Ismail;Manal F. Elshamaa(Department of Pediatrics, National Research Centre, Cairo, Egypt;Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt;Departments of Clinical Pathology, National Research Centre, Cairo, Egypt;Oncology Department, Faculty of Medicine, Cairo University, Cairo, Egypt)

机构地区：[1]Department of Pediatrics, National Research Centre, Cairo, Egypt [2]Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt [3]Departments of Clinical Pathology, National Research Centre, Cairo, Egypt [4]Oncology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

出　　处：《Open Journal of Nephrology》2021年第3期422-436,共15页肾脏病（英文）

摘　　要：<strong>Backgrounds:</strong> Recent advances in post Kidney Transplantation (KT) care, have led to a dramatic improvement in short-term outcomes in order to achieve transplantation tolerance;including the ideal tool for clinical monitoring & new therapeutic line. This study was undertaken to analyze the CD62L in Kidney Transplant Recipients (KTRs) and to investigate its efficacy as a marker of good graft survival. <strong>Methods:</strong> Fifty pediatric KTRs and 12 healthy controls were included in the study, the frequency of T cell activation markers;CD62L was measured with flow cytometry after renal transplantation. Clinical, laboratory, immunosuppressive therapy data and graft function of transplant recipients were collected and correlated with their CD62L peripheral blood percentage. <strong>Results:</strong> The circulating CD62L% was significantly more in transplant recipients than controls (44.74% ± 17.45% vs. 33.36% ± 11.54%, p = 0.02). CD 62L% was more frequent in recipients of living related donors (p = 0.05), positively correlated with donor age (p = 0.04, r = -0.29^*) and CD 4% (p = 0.000, r = 0.615). CD26L% did not show significant association with acute rejection or chronic rejection (p = 0.432, p = 0.91 respectively) or with graft function (serum creatinine or eGFR, p = 0.086, p = 0.988 respectively) or immunosuppressive medications. <strong>Conclusion:</strong> Peripheral CD62L% is increased after KT than healthy controls, however, it cannot reflect either clinical (serum creatinine and eGFR) or pathological renal graft injury. CD62L surface marker needs more analysis for its potential diagnostic and therapeutic implications as a Treg cell activation marker.<strong>Backgrounds:</strong> Recent advances in post Kidney Transplantation (KT) care, have led to a dramatic improvement in short-term outcomes in order to achieve transplantation tolerance;including the ideal tool for clinical monitoring & new therapeutic line. This study was undertaken to analyze the CD62L in Kidney Transplant Recipients (KTRs) and to investigate its efficacy as a marker of good graft survival. <strong>Methods:</strong> Fifty pediatric KTRs and 12 healthy controls were included in the study, the frequency of T cell activation markers;CD62L was measured with flow cytometry after renal transplantation. Clinical, laboratory, immunosuppressive therapy data and graft function of transplant recipients were collected and correlated with their CD62L peripheral blood percentage. <strong>Results:</strong> The circulating CD62L% was significantly more in transplant recipients than controls (44.74% ± 17.45% vs. 33.36% ± 11.54%, p = 0.02). CD 62L% was more frequent in recipients of living related donors (p = 0.05), positively correlated with donor age (p = 0.04, r = -0.29^*) and CD 4% (p = 0.000, r = 0.615). CD26L% did not show significant association with acute rejection or chronic rejection (p = 0.432, p = 0.91 respectively) or with graft function (serum creatinine or eGFR, p = 0.086, p = 0.988 respectively) or immunosuppressive medications. <strong>Conclusion:</strong> Peripheral CD62L% is increased after KT than healthy controls, however, it cannot reflect either clinical (serum creatinine and eGFR) or pathological renal graft injury. CD62L surface marker needs more analysis for its potential diagnostic and therapeutic implications as a Treg cell activation marker.

关 键 词：CD62L Regulatory T Cells TRANSPLANTATION Graft Survival CHILDREN 

分 类 号：R73[医药卫生—肿瘤]