Differential IFN-Gamma (IFN-γ), Interleukin 10 (IL-10) and Cardiac Troponin I (cTnI) Responses in Natural Bovine Trypanosomosis in Nigeria  

作　　者：Michael I. Takeet Benjamin O. Fagbemi Sunday O. Peters Matthew Wheto Abdulmojeed Yakubu Marcos DeDonato Ikhide G. Imumorin Michael I. Takeet;Benjamin O. Fagbemi;Sunday O. Peters;Matthew Wheto;Abdulmojeed Yakubu;Marcos DeDonato;Ikhide G. Imumorin(Animal Genetics and Genomics Laboratory, International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA;Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, Nigeria;Department of Veterinary Microbiology and Parasitology, University of Ibadan, Ibadan, Nigeria;Department of Animal Science, Berry College, Mount Berry, GA, USA;Department of Animal and Dairy Sciences, University of Georgia, Athens, GA, USA;Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria;Department of Animal Science, Nasarawa State University, Lafia, Nigeria;Department of Biomedicine, Universidad de Oriente, Cumana, Venezuela)

机构地区：[1]Animal Genetics and Genomics Laboratory, International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA [2]Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, Nigeria [3]Department of Veterinary Microbiology and Parasitology, University of Ibadan, Ibadan, Nigeria [4]Department of Animal Science, Berry College, Mount Berry, GA, USA [5]Department of Animal and Dairy Sciences, University of Georgia, Athens, GA, USA [6]Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria [7]Department of Animal Science, Nasarawa State University, Lafia, Nigeria [8]Department of Biomedicine, Universidad de Oriente, Cumana, Venezuela

出　　处：《Open Journal of Veterinary Medicine》2016年第7期105-111,共8页兽医学（英文）

摘　　要：Trypanosomosis is major drawback to profitable livestock production in sub-Sahara African, including Nigeria. Knowledge of the cytokines production in the phase of natural infection may help to better diagnose, treat and prevent bovine trypanosomosis. The purpose of the this study was to determine the levels of interferon-gamma (IFN-γ), interleukin-10 (IL-10) and cardiac troponin–I (cTnI) in the sera of cattle naturally infected with T. brucei, T. congolense and T. vivax and correlate these levels with parasitaemia and PCV of the infected animals. Five milliliter of blood samples were collected via the jugular vein from 411 randomly selected cattle into EDTA and non-citrated bottle. PCV was determined manually using HCT. Trypansomes were detected and characterized by microscopy and PCR, respectively. Serum levels of IFN-γ, IL-10 and cTnI were determined using commercial ELISA kit. Data were summarized using descriptive statistic and significance of differences determined by ANOVA. Of the 62 samples positive for trypanosomes by microscopy, 50 samples were confirmed to species level by PCR. The sera levels of IFN-γ, IL-10 and cTnI of infected cattle were higher than non-infected cattle. The differences were not significant (p γ, IL-10 and cTnI in cattle with natural trypanosomosis. Further investigation is required to understand the specific effect of trypanosomes on myocardiac integrity and interaction between the two cytokines in natural trypanosomosis in cattle.Trypanosomosis is major drawback to profitable livestock production in sub-Sahara African, including Nigeria. Knowledge of the cytokines production in the phase of natural infection may help to better diagnose, treat and prevent bovine trypanosomosis. The purpose of the this study was to determine the levels of interferon-gamma (IFN-γ), interleukin-10 (IL-10) and cardiac troponin–I (cTnI) in the sera of cattle naturally infected with T. brucei, T. congolense and T. vivax and correlate these levels with parasitaemia and PCV of the infected animals. Five milliliter of blood samples were collected via the jugular vein from 411 randomly selected cattle into EDTA and non-citrated bottle. PCV was determined manually using HCT. Trypansomes were detected and characterized by microscopy and PCR, respectively. Serum levels of IFN-γ, IL-10 and cTnI were determined using commercial ELISA kit. Data were summarized using descriptive statistic and significance of differences determined by ANOVA. Of the 62 samples positive for trypanosomes by microscopy, 50 samples were confirmed to species level by PCR. The sera levels of IFN-γ, IL-10 and cTnI of infected cattle were higher than non-infected cattle. The differences were not significant (p γ, IL-10 and cTnI in cattle with natural trypanosomosis. Further investigation is required to understand the specific effect of trypanosomes on myocardiac integrity and interaction between the two cytokines in natural trypanosomosis in cattle.

关 键 词：CATTLE Cardiac Troponin INTERFERON-GAMMA INTERLEUKIN-10 TRYPANOSOMOSIS 

分 类 号：R54[医药卫生—心血管疾病]