Topical Application of Cudrania tricuspidata Stem Extract Inhibits Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model: An Experimental Animal Study  

作　　者：Yoo-Sin Park Shin-Hee Kim Sang-Yeon Kim Gae-Myoung Koh Ju-Hwan Suh Ju-Seop Kang Yoo-Sin Park;Shin-Hee Kim;Sang-Yeon Kim;Gae-Myoung Koh;Ju-Hwan Suh;Ju-Seop Kang(Division of Molecular Therapeutics Development, Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea;Department of Clinical Nutrition, Shintong Rehabilitation Clinic, Yongin, Republic of Korea;Department of Food & Nutrition, Hanyang Women’s University, Seoul, Republic of Korea;Department of Pharmacology, College of Medicine, Hanyang University, Seoul, Republic of Korea;Institute of Medical Science, Hanyang University, Seoul, Republic of Korea;Department of New Drug Development, College of Medicine, Hanyang University, Seoul, Republic of Korea;Department of Biology, Johns Hopkins University, Maryland, USA;Department of Bioengineering, College of Engineering, Hanyang University, Seoul, Republic of Korea)

机构地区：[1]Division of Molecular Therapeutics Development, Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea [2]Department of Clinical Nutrition, Shintong Rehabilitation Clinic, Yongin, Republic of Korea [3]Department of Food & Nutrition, Hanyang Women’s University, Seoul, Republic of Korea [4]Department of Pharmacology, College of Medicine, Hanyang University, Seoul, Republic of Korea [5]Institute of Medical Science, Hanyang University, Seoul, Republic of Korea [6]Department of New Drug Development, College of Medicine, Hanyang University, Seoul, Republic of Korea [7]Department of Biology, Johns Hopkins University, Maryland, USA [8]Department of Bioengineering, College of Engineering, Hanyang University, Seoul, Republic of Korea

出　　处：《Pharmacology & Pharmacy》2016年第8期358-367,共10页药理与制药（英文）

摘　　要：Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by elevated immunoglobulin E (IgE), mast cell infiltration and skin lesions including pruritus, erythema and eczema. Cudrania tricuspidata extracts have been clinically administered for a long time in the East Asia including Korean and China as a home-remedy to diminish the inflammation of gastritis and hepatitis. To examine whether it works on AD or not, an AD-like animal model was experimented in this study. AD was induced by applying Dermatophagoides farinae (D. farinae) extract to the backs of 9-week old NC/Nga mice for 21 days. Following this, an ethanol extract of C. tricuspidata stems (EECT) was applied topically for 14 days to the sensitized skin, while distilled water was used as a control (EECT0 mice). Anti-AD effects of EECT were evaluated using scores for AD-like skin lesions, serum IgE levels and mast cell counts in the skin dermal layers to assess inflammation. Topically applied ethanol extract of Cudrania tricuspidata stems (EECT 7.5, 25 and 75 mg/mL) markedly reduced AD-like skin lesions after 4 days (by 30.1%, 31.4% and 38.5%, respectively) and also after 14 days (by 63.6%, 66.1% and 49.6%, respectively), while distilled water improved AD by 17.8% and 38.7%, respectively (p D. farinae extract (p = 0.003) and EECT attenuated the mast cell overproduction, and reduced mast cell degranulation markedly. Attenuation was most obvious in the early stage of EECT treatment when the AD was most acute.Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by elevated immunoglobulin E (IgE), mast cell infiltration and skin lesions including pruritus, erythema and eczema. Cudrania tricuspidata extracts have been clinically administered for a long time in the East Asia including Korean and China as a home-remedy to diminish the inflammation of gastritis and hepatitis. To examine whether it works on AD or not, an AD-like animal model was experimented in this study. AD was induced by applying Dermatophagoides farinae (D. farinae) extract to the backs of 9-week old NC/Nga mice for 21 days. Following this, an ethanol extract of C. tricuspidata stems (EECT) was applied topically for 14 days to the sensitized skin, while distilled water was used as a control (EECT0 mice). Anti-AD effects of EECT were evaluated using scores for AD-like skin lesions, serum IgE levels and mast cell counts in the skin dermal layers to assess inflammation. Topically applied ethanol extract of Cudrania tricuspidata stems (EECT 7.5, 25 and 75 mg/mL) markedly reduced AD-like skin lesions after 4 days (by 30.1%, 31.4% and 38.5%, respectively) and also after 14 days (by 63.6%, 66.1% and 49.6%, respectively), while distilled water improved AD by 17.8% and 38.7%, respectively (p D. farinae extract (p = 0.003) and EECT attenuated the mast cell overproduction, and reduced mast cell degranulation markedly. Attenuation was most obvious in the early stage of EECT treatment when the AD was most acute.

关 键 词：Atopic Dermatitis Cudrania tricuspidata Stem Dermatophagoides farina Immunoglobulin E (IgE) NC/Nga Mouse 

分 类 号：R73[医药卫生—肿瘤]