Purification and Some Properties of Endo-1,4-β-Glucanases of Trichoderma harzianum UzCF-28  

作　　者：N. Sh. Azimova D. M. Khamidov M. B. Djumagulov Z. S. Shakirov N. Sh. Azimova;D. M. Khamidov;M. B. Djumagulov;Z. S. Shakirov(Institute of Microbiology of Uzbekistan Academy of Sciences, Tashkent, Republic of Uzbekistan)

机构地区：[1]Institute of Microbiology of Uzbekistan Academy of Sciences, Tashkent, Republic of Uzbekistan

出　　处：《Open Journal of Applied Sciences》2016年第8期514-523,共11页应用科学（英文）

摘　　要：This work aimed at isolation, purification and study of biochemical features of cellulolytic enzymes synthesized by Trichoderma harzianum UzCF-28 strain. Strain UzCF-28 revealed a high cellulolytic activity during submerged cultivation in the liquid culture on modified Mandels nutrient medium, where wheat straw was used as a source of carbon. As a result of purification by precipitation with ammonium sulfate and further ion exchange chromatography, two isoforms of endo- 1,4-β-glucanase-EG II and EG III with molecular weight of 135 and 75 kDa respectively were revealed. The pH optimum for EG I and EG III was 4.5, while for EG II—4.7, irrespective of the applied substrates—either CMC or “Whatman filter” paper. Heating up to 40°C of EG III did not lead to its inactivation, and on the contrary, its activity increased by more than three times comparing to the initial activity of the enzyme, i.e. thermostability of EG III among tested enzymes significantly varied.This work aimed at isolation, purification and study of biochemical features of cellulolytic enzymes synthesized by Trichoderma harzianum UzCF-28 strain. Strain UzCF-28 revealed a high cellulolytic activity during submerged cultivation in the liquid culture on modified Mandels nutrient medium, where wheat straw was used as a source of carbon. As a result of purification by precipitation with ammonium sulfate and further ion exchange chromatography, two isoforms of endo- 1,4-β-glucanase-EG II and EG III with molecular weight of 135 and 75 kDa respectively were revealed. The pH optimum for EG I and EG III was 4.5, while for EG II—4.7, irrespective of the applied substrates—either CMC or “Whatman filter” paper. Heating up to 40°C of EG III did not lead to its inactivation, and on the contrary, its activity increased by more than three times comparing to the initial activity of the enzyme, i.e. thermostability of EG III among tested enzymes significantly varied.

关 键 词：Trichoderma harzianum ENZYME Endo-1 4-β-Glucanase PURIFICATION 

分 类 号：O64[理学—物理化学]