Production of Endopolysaccharides from Malaysia’s Local Mushrooms in Air-Lift Bioreactor  

作　　者：Shaiful Azuar Mohamad Mat Rasol Awang Rusli Ibrahim Choong Yew Keong Mohd Yusof Hamzah Rosnani Abdul Rashid Sobri Hussein Khairuddin Abdul Rahim Fauzi Daud Aidil Abdul Hamid Wan Mohtar Wan Yusoff 

机构地区：[1]Agrotechnology and Biosciences Division, Malaysian Nuclear Agency, Bangi, Malaysia [2]Herbal Medicine Research Centre, Institute For Medical Research, Kuala Lumpur, Malaysia [3]Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia

出　　处：《Advances in Bioscience and Biotechnology》2015年第7期456-462,共7页生命科学与技术进展（英文）

摘　　要：Four local mushroom species, viz. Auricularis polytricha, Lentinus edodes, Agrocybe sp and Pleurotus flabellatus were grown under submerged culture and screened for endopolysaccharides. The fermentation was done in 250 ml working volume Erlenmeyer flask and the fermentation curves for all species were established. Pleurotus flabellatus has the highest rate of biomass production at the rate of 0.180 g/L/day, at 10 days hence chosen for further investigation. Two additional media, viz. Mushroom Complete Media (MCM) and Yeast Malt (YM) were selected to be compared with potato extract(PE) media used initially. MCM media produced the highest biomass productivity at the rate of 0.311 g/L/day. Pleurotus flabellatus biomass was extracted using modified Mizuno method and the endopolysaccharide obtained was tested for β-glucan. The yield of β-glucan was 7.70 ± 1.11 g/100g. The polysaccharides were purified using column chromatography to yield four fractions. The fourth fraction F4, gave the highest molecular weight at 3.058 × 106 Dalton (11.8%) and 1.282 × 104 Dalton (88.2%). The mushroom, P. flabbelatus was cultured using air-lift bioreactor, and the highest productivity was obtained at air-flowrate 2 L/min, yielding 2.25 g/L/day. The yield of biomass against substrate used (glucose consumption) Yb/s was 0.78 g/g.Four local mushroom species, viz. Auricularis polytricha, Lentinus edodes, Agrocybe sp and Pleurotus flabellatus were grown under submerged culture and screened for endopolysaccharides. The fermentation was done in 250 ml working volume Erlenmeyer flask and the fermentation curves for all species were established. Pleurotus flabellatus has the highest rate of biomass production at the rate of 0.180 g/L/day, at 10 days hence chosen for further investigation. Two additional media, viz. Mushroom Complete Media (MCM) and Yeast Malt (YM) were selected to be compared with potato extract(PE) media used initially. MCM media produced the highest biomass productivity at the rate of 0.311 g/L/day. Pleurotus flabellatus biomass was extracted using modified Mizuno method and the endopolysaccharide obtained was tested for β-glucan. The yield of β-glucan was 7.70 ± 1.11 g/100g. The polysaccharides were purified using column chromatography to yield four fractions. The fourth fraction F4, gave the highest molecular weight at 3.058 × 106 Dalton (11.8%) and 1.282 × 104 Dalton (88.2%). The mushroom, P. flabbelatus was cultured using air-lift bioreactor, and the highest productivity was obtained at air-flowrate 2 L/min, yielding 2.25 g/L/day. The yield of biomass against substrate used (glucose consumption) Yb/s was 0.78 g/g.

关 键 词：SUBMERGED Culture FERMENTATION MUSHROOM Β-GLUCAN Column Chromatography Molecular Weight 

分 类 号：R73[医药卫生—肿瘤]