Properties of Bacillus species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate  

作　　者：Frank Anayo Orji Ekaette Nduka Dike Adekunle Kolawole Lawal Abdulateef Omoniyi Sadiq Yewande Suberu Ariyo Caleb Famotemi Agatha Isioma Ugbana Folake Fashola Blossom Ita Samuel Olakiitan Olatope Emoleila Ejiya Itoandoan Abimbola Olajumoke Adefiranye Gloria Nwakaegho Elemo Frank Anayo Orji;Ekaette Nduka Dike;Adekunle Kolawole Lawal;Abdulateef Omoniyi Sadiq;Yewande Suberu;Ariyo Caleb Famotemi;Agatha Isioma Ugbana;Folake Fashola;Blossom Ita;Samuel Olakiitan Olatope;Emoleila Ejiya Itoandoan;Abimbola Olajumoke Adefiranye;Gloria Nwakaegho Elemo(Department of Biotechnology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria;Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria;Department of Food Technology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria)

机构地区：[1]Department of Biotechnology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria [2]Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria [3]Department of Food Technology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria

出　　处：《Advances in Bioscience and Biotechnology》2016年第3期142-148,共7页生命科学与技术进展（英文）

摘　　要：The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223 cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria using local raw materials such as Brewers Spent Grain reduces enzyme cost;cost of finished products and increases gross domestic products.The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223 cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria using local raw materials such as Brewers Spent Grain reduces enzyme cost;cost of finished products and increases gross domestic products.

关 键 词：CELLULASE Bacillus species Crude Activities Temperature pH Tolerance Brewers Spent Grains (BSG) 

分 类 号：G63[文化科学—教育学]