Effect of Wild Marjoram (<i>Origanum vulgare</i>) Plant Extracts on Capacitation of Sheep Spermatozoa <i>in Vitro</i>  

作　　者：Ebtesam S. Alenezy Ibrahim A. H. Barakat Nawal M. Al Musayeib 

机构地区：[1]Zoology Department, College of Science, King Saud University, Riyadh, KSA [2]Department of Biology, College of Science, Hafr Al Batin University, Hafr Al Batin, Saudi Arabia [3]Cell Biology Department, National Research Center, Giza, Egypt [4]Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

出　　处：《Advances in Bioscience and Biotechnology》2019年第4期82-97,共16页生命科学与技术进展（英文）

摘　　要：The purpose of this study was to evaluate the effect of the addition of Origanum vulgare extract to in vitro capacitation sperm medium (IVCSM). This study investigated the antioxidant and antimicrobial effects of O. vulgare extracts at different concentrations (0.3, 0.6, 1.2 μg/ml and 25.0, 50.0, 100.0 μg/ml, respectively) in IVCSM. Significant enhancements in semen quality parameters such as total motility, live and live capacitated sperm were found when O. vulgare extract was added as an antioxidant source (1.2 μg/ml). The treatment of spermatozoa with O. vulgare extract at the highest concentration (100 μg/ml) for 2 hrs without antibiotics improved sperm characteristics. In conclusion, incubation of sperm with O. vulgare extract in capacitation medium had beneficial effects on the characteristics of ram sperm.The purpose of this study was to evaluate the effect of the addition of Origanum vulgare extract to in vitro capacitation sperm medium (IVCSM). This study investigated the antioxidant and antimicrobial effects of O. vulgare extracts at different concentrations (0.3, 0.6, 1.2 μg/ml and 25.0, 50.0, 100.0 μg/ml, respectively) in IVCSM. Significant enhancements in semen quality parameters such as total motility, live and live capacitated sperm were found when O. vulgare extract was added as an antioxidant source (1.2 μg/ml). The treatment of spermatozoa with O. vulgare extract at the highest concentration (100 μg/ml) for 2 hrs without antibiotics improved sperm characteristics. In conclusion, incubation of sperm with O. vulgare extract in capacitation medium had beneficial effects on the characteristics of ram sperm.

关 键 词：SHEEP Sperm ORIGANUM vulgare In Vitro 

分 类 号：R73[医药卫生—肿瘤]