Molecular Characterization and Technological Properties of Lactic Acid Bacteria, Bacillus and Yeast of Probiotic Interest Isolated from Fermented Porridges  

作　　者：Boureima Kagambèga Namwin Siourimè Somda Hama Cissé Oumarou Zongo Yves Traoré Aly Savadogo Boureima Kagambèga;Namwin Siourimè Somda;Hama Cissé;Oumarou Zongo;Yves Traoré;Aly Savadogo(Laboratoire de Biochimie et Immunologie Appliquées (LABIA), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso;Centre National de la Recherche Scientifique et Technologique (CNRST)/IRSAT/Département Technologie Alimentaire (DTA)/Direction Régionale de l’Ouest, Bobo-Dioulasso, Burkina Faso;Unité de Formation et de Recherche en Sciences et Techniques (UFR/ST), Université Thomas SANKARA, Ouagadougou, Burkina Faso)

机构地区：[1]Laboratoire de Biochimie et Immunologie Appliquées (LABIA), Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso [2]Centre National de la Recherche Scientifique et Technologique (CNRST)/IRSAT/Département Technologie Alimentaire (DTA)/Direction Régionale de l’Ouest, Bobo-Dioulasso, Burkina Faso [3]Unité de Formation et de Recherche en Sciences et Techniques (UFR/ST), Université Thomas SANKARA, Ouagadougou, Burkina Faso

出　　处：《Advances in Bioscience and Biotechnology》2022年第7期284-297,共14页生命科学与技术进展（英文）

摘　　要：Cereal-based porridges are among fermented foods with a composite microbiota. The objective of this work is to characterize the microbiota of porridge. Samples of porridge were collected in Ouagadougou and analyzed according to standard methods in microbiology. The presumed strains obtained were characterized by polymerase chain reaction (PCR). Technological abilities were estimated by tests for resistance to acid pH, bile, antibiotics and antimicrobial, proteolytic and lipolytic activities. All presumptive Bacillus, lactic acid bacteria and yeast were characterized by PCR. Four (BC1a;BC9b;BC2a and BC1b) strains were confirmed to Bacillus by PCR, 6 strains to Lactobacillus and only to Saccharomyces cerevisiae. All strains were sensitive to two antibiotics gentamycin and imipenem. In contrast, all strains were resistant to oxacillin, amoxicillin-clavulanic acid, streptomycin, penicillin and ticarcillin. Strains tested were resistant to bile but in terms of pH this resistance was relative. Potential probiotic strains have been shown to be effective in inhibiting pathogens. Proteolytic and lipolytic activities were positive on all strains. The characterization of strains, although concerned with a non-exhaustive list of primers, has made it possible to confirm the strains that may be of good quality probiotics if a quantitative study is carried out on technological aptitudes.Cereal-based porridges are among fermented foods with a composite microbiota. The objective of this work is to characterize the microbiota of porridge. Samples of porridge were collected in Ouagadougou and analyzed according to standard methods in microbiology. The presumed strains obtained were characterized by polymerase chain reaction (PCR). Technological abilities were estimated by tests for resistance to acid pH, bile, antibiotics and antimicrobial, proteolytic and lipolytic activities. All presumptive Bacillus, lactic acid bacteria and yeast were characterized by PCR. Four (BC1a;BC9b;BC2a and BC1b) strains were confirmed to Bacillus by PCR, 6 strains to Lactobacillus and only to Saccharomyces cerevisiae. All strains were sensitive to two antibiotics gentamycin and imipenem. In contrast, all strains were resistant to oxacillin, amoxicillin-clavulanic acid, streptomycin, penicillin and ticarcillin. Strains tested were resistant to bile but in terms of pH this resistance was relative. Potential probiotic strains have been shown to be effective in inhibiting pathogens. Proteolytic and lipolytic activities were positive on all strains. The characterization of strains, although concerned with a non-exhaustive list of primers, has made it possible to confirm the strains that may be of good quality probiotics if a quantitative study is carried out on technological aptitudes.

关 键 词：BACILLUS Lactic Bacteria YEAST Porridge Probiotic 

分 类 号：O62[理学—有机化学]