Transgenerational and Sexual Auto-Dissemination Within Anopheles Mosquitoes of the Malaria Parasite Transmission Blocking Microsporidia Sp MB in Burkina Faso  

作　　者：Souro A. Millogo Etienne Bilgo Issiaka Saré Doubé L. Lamy Edounou Jacques Gnambani Maurice Konkobo Gaston A. M. Belem Abdoulaye Diabaté Souro A. Millogo;Etienne Bilgo;Issiaka Saré;Doubé L. Lamy;Edounou Jacques Gnambani;Maurice Konkobo;Gaston A. M. Belem;Abdoulaye Diabaté(Institut de Recherche en Sciences de la Sant Direction Rgionale de lOuest (IRSS/DRO), Centre National de Recherche des Sciences et Technologies (CNRST), Bobo-Dioulasso, Burkina Faso;Ecole Doctorale des Sciences Naturelles et Agronomie (ED-SNA), Universit Nazi BONI, Bobo-Dioulasso, Burkina Faso;Institut Nationale de Sant Publique (INSP), Centre Muraz, Bobo-Dioulasso, Burkina Faso;Laboratoire d’Entomologie Fondamentale et Applique (LEFA), Universit Joseph Ki-ZERBO, Ougadougou, Burkina Faso)

机构地区：[1]Institut de Recherche en Sciences de la Sant Direction Rgionale de lOuest (IRSS/DRO), Centre National de Recherche des Sciences et Technologies (CNRST), Bobo-Dioulasso, Burkina Faso [2]Ecole Doctorale des Sciences Naturelles et Agronomie (ED-SNA), Universit Nazi BONI, Bobo-Dioulasso, Burkina Faso [3]Institut Nationale de Sant Publique (INSP), Centre Muraz, Bobo-Dioulasso, Burkina Faso [4]Laboratoire d’Entomologie Fondamentale et Applique (LEFA), Universit Joseph Ki-ZERBO, Ougadougou, Burkina Faso

出　　处：《Advances in Bioscience and Biotechnology》2024年第11期655-673,共19页生命科学与技术进展（英文）

摘　　要：Introduction: Malaria control needs the development of complementary and/or alternative strategies such as biological controls. Despite, malaria’s current control efforts, the spread and the emergence of insecticide resistance in vectors undermine the fight against vectors. Endonsymbiotic fungi can be a good candidate to include in the existence of Arsenal. However, we know little about endosymbiotic fungi transmission and its impact on malaria transmission. In this paper, the authors aimed to investigate Microsporidia sp MB transgenerational and sexual autodissemination and malaria parasites within Anopheline mosquitoes. Methods: An entomology survey was conducted in Burkina Faso for one year (June 2020 to June 2021) using the Residual Fauna Capture method. Infection rates in collected females, sexual autodissemination of Microsporidia sp MB in both Microsporidia sp MB negative females and Microsporidia sp MB negative males through mating, transgenerational prevalence from parents to offsprings and the correlation between Microsporidia sp MB and Plasmodium falciparum were investigated. Results: Results show Microsporidia sp MB infection in An. gambaie s.l in Burkina Faso. The prevalence was significantly higher (21.78%) in An. coluzzii than An. gambiae s.s (16.89%) (p-value = 0.03). Sexual auto-dissemination of Microsporidia sp MB in Microsporidia sp MB uninfected females was significantly 3-fold lower than those in Microsporidia sp MB uninfected males (9.23% and 33.33%, p-value = 0.03) during mating for An. coluzzii lines. Microsporidia sp MB prevalence was significantly higher through mosquitos’ generations in An. gambaie s.s than An. coluzzii (30.23% vs 26.41%, p-value Microsporidia sp MB and Plasmodium infection rate with 73% of Microsporidia sp MB positive mosquitoes which were negative at Plasmodium infection (p-value Conclusions: Our findings pave the road to developing new malaria control technologies by making Microsporidia sp MB-positive males sexually competitive with wild males to spread the fungIntroduction: Malaria control needs the development of complementary and/or alternative strategies such as biological controls. Despite, malaria’s current control efforts, the spread and the emergence of insecticide resistance in vectors undermine the fight against vectors. Endonsymbiotic fungi can be a good candidate to include in the existence of Arsenal. However, we know little about endosymbiotic fungi transmission and its impact on malaria transmission. In this paper, the authors aimed to investigate Microsporidia sp MB transgenerational and sexual autodissemination and malaria parasites within Anopheline mosquitoes. Methods: An entomology survey was conducted in Burkina Faso for one year (June 2020 to June 2021) using the Residual Fauna Capture method. Infection rates in collected females, sexual autodissemination of Microsporidia sp MB in both Microsporidia sp MB negative females and Microsporidia sp MB negative males through mating, transgenerational prevalence from parents to offsprings and the correlation between Microsporidia sp MB and Plasmodium falciparum were investigated. Results: Results show Microsporidia sp MB infection in An. gambaie s.l in Burkina Faso. The prevalence was significantly higher (21.78%) in An. coluzzii than An. gambiae s.s (16.89%) (p-value = 0.03). Sexual auto-dissemination of Microsporidia sp MB in Microsporidia sp MB uninfected females was significantly 3-fold lower than those in Microsporidia sp MB uninfected males (9.23% and 33.33%, p-value = 0.03) during mating for An. coluzzii lines. Microsporidia sp MB prevalence was significantly higher through mosquitos’ generations in An. gambaie s.s than An. coluzzii (30.23% vs 26.41%, p-value Microsporidia sp MB and Plasmodium infection rate with 73% of Microsporidia sp MB positive mosquitoes which were negative at Plasmodium infection (p-value Conclusions: Our findings pave the road to developing new malaria control technologies by making Microsporidia sp MB-positive males sexually competitive with wild males to spread the fung

关 键 词：TRANSGENERATIONAL Sexual Autodissemination Correlation Prevalence Microsporidia Sp MB ANOPHELES Plasmodium Falciparum Malaria Burkina Faso 

分 类 号：R53[医药卫生—内科学]