Rapid Detection Tool for Vancomycin-Resistant <i>Enterococcus</i>(VRE) Directly from Human Specimens  

作　　者：Stephen C. Edberg 

机构地区：[1]Mt. Sinai Health System, New York, NY, USA

出　　处：《Advances in Microbiology》2014年第12期835-838,共4页微生物学（英文）

摘　　要：A new, simple, and one-step tool for the direct detection of vancomycin-resistant Enterocococcus (VRE) EPI-V&reg;(Pilots Point LLC, Sarasota, FL) is presented. It contains all the ingredients in a unique stable powder form in a standard test tube. One only needs to add water, inoculate the specimen, and incubate. Specimens consisted of 553 sequential human rectal/perirectal swabs for VRE surveillance. The presence of VRE was denoted by the production of two sequential color changes corresponding to growth in bile-esculin and production of a positive PYR reaction. The EPI-V&reg;tool was compared to reference VRE detection methods. EPI-V&reg;showed a sensitivity of ?102% and a specificity of 98.4% for the detection of VRE. The EPI-V&reg;tool offers significant advantages: no skilled technologist time required, simple quality control, highly conserved incubator and refrigerator space, and low cost.A new, simple, and one-step tool for the direct detection of vancomycin-resistant Enterocococcus (VRE) EPI-V&reg;(Pilots Point LLC, Sarasota, FL) is presented. It contains all the ingredients in a unique stable powder form in a standard test tube. One only needs to add water, inoculate the specimen, and incubate. Specimens consisted of 553 sequential human rectal/perirectal swabs for VRE surveillance. The presence of VRE was denoted by the production of two sequential color changes corresponding to growth in bile-esculin and production of a positive PYR reaction. The EPI-V&reg;tool was compared to reference VRE detection methods. EPI-V&reg;showed a sensitivity of ?102% and a specificity of 98.4% for the detection of VRE. The EPI-V&reg;tool offers significant advantages: no skilled technologist time required, simple quality control, highly conserved incubator and refrigerator space, and low cost.

关 键 词：VRE STOOL Carriage Detection 

分 类 号：R73[医药卫生—肿瘤]