The <i>PafR</i>Gene Is Required for Antifungal Activity of Strain MS82 against <i>Mycogone perniciosa</i>  

作　　者：Lin Ma Xiaoqiang Wang Peng Deng Sonya M. Baird Youzhou Liu Shaoxuan Qu Shi-En Lu 

机构地区：[1]Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China [2]Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, USA [3]Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China

出　　处：《Advances in Microbiology》2017年第4期217-230,共14页微生物学（英文）

摘　　要：Bacterial strain MS82, isolated from the rhizosphere of a soybean plant, belongs to the species Pseudomonas fluorescens. The most important feature of strain MS82 is the production of antifungal activity against the mushroom pathogenic fungus Mycogone perniciosa but not against the mushroom fungus Agaricus bisporus. In this study, the mutant MS82MT19 generated with the EZ-Tn5 transposon system completely lost the antifungal activity against M. perniciosa. An open-reading frame named as PafR and predicted to code for a sensory box GGDEF/EAL domain protein, was disrupted in MS82MT19. To further confirm the function of this gene, site-directed mutagenesis with insertion of the terminatorless nptII cassette into the PafR gene was used to generate the mutant MS82SD19. As expected, there was no detectable antifungal activity of mutant MS82SD19 against M. perniciosa. These results suggest that the PafR gene plays an important role in the production of antifungal activity of P. fluorescens strain MS82.Bacterial strain MS82, isolated from the rhizosphere of a soybean plant, belongs to the species Pseudomonas fluorescens. The most important feature of strain MS82 is the production of antifungal activity against the mushroom pathogenic fungus Mycogone perniciosa but not against the mushroom fungus Agaricus bisporus. In this study, the mutant MS82MT19 generated with the EZ-Tn5 transposon system completely lost the antifungal activity against M. perniciosa. An open-reading frame named as PafR and predicted to code for a sensory box GGDEF/EAL domain protein, was disrupted in MS82MT19. To further confirm the function of this gene, site-directed mutagenesis with insertion of the terminatorless nptII cassette into the PafR gene was used to generate the mutant MS82SD19. As expected, there was no detectable antifungal activity of mutant MS82SD19 against M. perniciosa. These results suggest that the PafR gene plays an important role in the production of antifungal activity of P. fluorescens strain MS82.

关 键 词：PSEUDOMONAS FLUORESCENS ANTIFUNGAL Activity Mycogone perniciosa MUTAGENESIS 

分 类 号：R73[医药卫生—肿瘤]