Portable Diagnostic Platform for Detection of Microorganisms Coliforms and E.coli  

作　　者：Natália Faria Romao Nathalia Aparecida Costa Pereira Maribel Elizabeth Funes-Huacca Luciana Gatto Brito 

机构地区：[1]Rede de Biodiversidade e Biotecnologia da Amazonia Legal(BIONORTE),Fundacao Universidade Federal de Rondonia(UNIR),Porto Velho,Brazil [2]Department of Chemistry,Fundacao Universidade Federal de Rondônia(UNIR),Porto Velho,Brazil [3]Empresa Brasileira de Pesquisa Agropecuária(EMBRAPA),Belém,Brazil

出　　处：《Advances in Microbiology》2020年第5期224-237,共14页微生物学（英文）

基　　金：This work was supported by the Empresa Brasileira de Pesquisa Agropecuária(EMBRAPA)and the Fundacao Universidade Federal de Rondônia(UNIR);specially to the Environmental Biogeochemistry Wolfgang C.Pfeiffer Research Group;which provided laboratory space and supplies for carrying out the research.The first author also thanks the Fundacao de AmparoàPesquisa de Rondônia(FAPERO)for the financial support granted through a scholarship(MSc and PhD scholarship program-MS/DR-CALL 010/2016).

摘　　要：Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.

关 键 词：Portable Device Bacterial Contamination Environment 

分 类 号：R73[医药卫生—肿瘤]