Fecal Metabolomes in Response to Feed Supplemented with Fermented <i>Parkia biglobosa</i>and <i>Sphenostylis stenocarpa</i>in Obese Rats  

作　　者：Olayinka Anthony Awoyinka Tolani Rachael Funmilola Comfort Oladele Margret Olutayo Alese Elijah Olalekan Odesanmi Daisi David Ajayi Gbenga Sunday Adeleye Bunmi Comfort Boyede Olayinka Anthony Awoyinka;Tolani Rachael;Funmilola Comfort Oladele;Margret Olutayo Alese;Elijah Olalekan Odesanmi;Daisi David Ajayi;Gbenga Sunday Adeleye;Bunmi Comfort Boyede(Department of Medical Biochemistry, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria;Department of Microbiology, Ekiti State University, Ado Ekiti, Nigeria;Department of Anatomy, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria;Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria;Department of Chemical Pathology, Ekiti State University Teaching Hospital, Ado Ekiti, Nigeria;Department of Physiology, Ekiti State University, Ado Ekiti, Nigeria)

机构地区：[1]Department of Medical Biochemistry, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria [2]Department of Microbiology, Ekiti State University, Ado Ekiti, Nigeria [3]Department of Anatomy, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria [4]Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria [5]Department of Chemical Pathology, Ekiti State University Teaching Hospital, Ado Ekiti, Nigeria [6]Department of Physiology, Ekiti State University, Ado Ekiti, Nigeria

出　　处：《Advances in Microbiology》2021年第2期63-74,共12页微生物学（英文）

摘　　要：The ubiquitous consumption of junk foods has drastically contributed to the exponential rise in the incidence of obesity. Hence, the present study explores the therapeutic effect of selected indigenous wild bean <i>Sphenostylis stenocarpa</i> (<i>Otili</i>) and condiment fermented <i>Parkia biglobosa</i> (<i>Iru</i>) on obese rats. The rats were fed with a high fat diet for four weeks and the gut microbiota was monitored every other day throughout the period of the experiment. Then, the fecal metabolome was analysed by Gas Chromatography Mass Spectroscopy (GC-MS). Although there was a decrease in the mean weight of rats treated with fermented <i>iru</i> compared with those given <i>Otili</i>, it was not statistically significantly (p ≤ 0.05). The organisms identified from the fecal samples of the fermented <i>Iru</i> groups are <i>Proteus vulgaris</i>, <i>Bacillus cereus</i> and <i>Esherichia coli</i> while those identified from the <i>Otili</i> group include <i>Escherichia coli</i>, and <i>Citrobacter Freundii</i>. However, further study revealed that <i>Otili</i> and <i>Iru</i> had a similar faecal metabolome. Medium chain fatty acids, such as Decanoic acid, Octanoic acid, ethyl tetradecanoate, Hexadecanoic acid, Methyl tetradecanoate, 9-Hexadecanoic acid, Hexadecnoic acid, cis-10-Hepadecanoic acid, are the most common compounds found in this study. This suggests the fact that the associated gut microbiota from breakdown of respective food samples must have actively mediated in their roles of ameliorating the effect of obesity.The ubiquitous consumption of junk foods has drastically contributed to the exponential rise in the incidence of obesity. Hence, the present study explores the therapeutic effect of selected indigenous wild bean <i>Sphenostylis stenocarpa</i> (<i>Otili</i>) and condiment fermented <i>Parkia biglobosa</i> (<i>Iru</i>) on obese rats. The rats were fed with a high fat diet for four weeks and the gut microbiota was monitored every other day throughout the period of the experiment. Then, the fecal metabolome was analysed by Gas Chromatography Mass Spectroscopy (GC-MS). Although there was a decrease in the mean weight of rats treated with fermented <i>iru</i> compared with those given <i>Otili</i>, it was not statistically significantly (p ≤ 0.05). The organisms identified from the fecal samples of the fermented <i>Iru</i> groups are <i>Proteus vulgaris</i>, <i>Bacillus cereus</i> and <i>Esherichia coli</i> while those identified from the <i>Otili</i> group include <i>Escherichia coli</i>, and <i>Citrobacter Freundii</i>. However, further study revealed that <i>Otili</i> and <i>Iru</i> had a similar faecal metabolome. Medium chain fatty acids, such as Decanoic acid, Octanoic acid, ethyl tetradecanoate, Hexadecanoic acid, Methyl tetradecanoate, 9-Hexadecanoic acid, Hexadecnoic acid, cis-10-Hepadecanoic acid, are the most common compounds found in this study. This suggests the fact that the associated gut microbiota from breakdown of respective food samples must have actively mediated in their roles of ameliorating the effect of obesity.

关 键 词：Microbiota Metabolomes MCFA SCFA OBESE 

分 类 号：O62[理学—有机化学]