Determination of the Population Structure of Fig Genotypes from Algeria and Turkey Using Inter Primer Binding Site-Retrotransposon and Simple Sequence Repeat Markers  

作　　者：Hakima Belttar Abdelouhab Yahia Seda Nemli Duygu Ates Semih Erdogmus Birgul Ertan Sara Himour Serra Hepaksoy M. Bahattin Tanyolac 

机构地区：[1]Department Biology and Plant Ecology, Faculty of Nature and Life Sciences, University Mentouri, Constantine, Algeria [2]Laboratory of Natural Sciences and Materials, Mila University Center, Algeria [3]Department of Genetics and Bioengineering, Gumushane University, Gumushane, Turkey [4]Department of Bioengineering, Ege University, Bornova-Izmir, Turkey [5]Ministry of Agriculture, Fig Research Institute, Aydı n, Turkey [6]Department of Horticulture, Ege University, Izmir, Turkey

出　　处：《Agricultural Sciences》2017年第12期1337-1357,共21页农业科学（英文）

摘　　要：In order to identify the variation and estimate the genetic diversity among the fig (Ficus carica L.) genotypes collected from Algeria and Turkey, the genetic relationships between 86 genotypes were investigated using 23 inter primer binding sites (iPBS)-retrotransposon and 16 simple sequence repeat (SSR) primers. A total of 63 polymorphic bands for the iPBS-retrotransposon markers and 25 alleles for the SSR markers were identified with an average of 2.7 and 1.6 per primer, respectively. The average value of polymorphism information content (PIC) for the iPBS markers (0.73) was higher than that for the SSR markers (0.69). Applying the neighbor-joining method to the combined iPBS-retrotransposon and SSR data, the fig genotypes were clustered into two groups. The STRUCTURE software was used to determine the population structure. Among the genotypes studied, two populations (K = 2) were identified indicating a low diversity between the Algerian and Turkish varieties. Both types of markers were able to differentiate all the fig genotypes and were efficient in discriminating the closely related genotypes. Our data also showed that as a universal marker, iPBS-retrotransposon is a useful tool for the molecular characterization of fig genotypes.In order to identify the variation and estimate the genetic diversity among the fig (Ficus carica L.) genotypes collected from Algeria and Turkey, the genetic relationships between 86 genotypes were investigated using 23 inter primer binding sites (iPBS)-retrotransposon and 16 simple sequence repeat (SSR) primers. A total of 63 polymorphic bands for the iPBS-retrotransposon markers and 25 alleles for the SSR markers were identified with an average of 2.7 and 1.6 per primer, respectively. The average value of polymorphism information content (PIC) for the iPBS markers (0.73) was higher than that for the SSR markers (0.69). Applying the neighbor-joining method to the combined iPBS-retrotransposon and SSR data, the fig genotypes were clustered into two groups. The STRUCTURE software was used to determine the population structure. Among the genotypes studied, two populations (K = 2) were identified indicating a low diversity between the Algerian and Turkish varieties. Both types of markers were able to differentiate all the fig genotypes and were efficient in discriminating the closely related genotypes. Our data also showed that as a universal marker, iPBS-retrotransposon is a useful tool for the molecular characterization of fig genotypes.

关 键 词：FICUS CARICA L. INTER PRIMER Binding Sites-Retrotransposon Simple Sequence Repeat Genetic Diversity Population Structure 

分 类 号：S5[农业科学—作物学]