Seed Quality Evaluation by Tetrazolium Staining of Parkia multijuga Benth  

作　　者：Marcelle Auday Costa Elizabeth Santos Cordeiro Shimizu Noemi Vianna Martins Leao Hugo Alves Pinheiro 

机构地区：[1]Departamento de Ciencias Florestais,Universidade Federal Rural da Amazonia,Belem,Brazil [2]Embrapa Amazonia Oriental,Belém,Brazil [3]Research and Technological Development of Universidade Federal Rural da Amazonia,Belém,Brazil

出　　处：《Agricultural Sciences》2018年第5期577-586,共10页农业科学（英文）

基　　金：Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(Brazilian National Council for Scientific and Technological Development)—CNPq for the research grant

摘　　要：The purpose of this study was to develop a staining pattern through tetrazolium test to evaluate seed viability of Parkia multijuga Benth (fava-arara-tucupi). The seeds were scarified, soaked into water and distilled for 48 hours for tegument removal and then immersed in a 0.05%;0.1%;and 0.3% tetrazolium solution for 6;18 and 24 hours at 35°C, in the dark. The evaluation was performed on the basis of stain intensity and aspect of the tissues. Based on color patterns, the seed images were classified in eight viability classes. Those results were compared with the germination test in a laboratory. The treatment in concentration of 0.1% for 6 hours was considered adequate for the specie, considering the adequate staining and time for analysis.The purpose of this study was to develop a staining pattern through tetrazolium test to evaluate seed viability of Parkia multijuga Benth (fava-arara-tucupi). The seeds were scarified, soaked into water and distilled for 48 hours for tegument removal and then immersed in a 0.05%;0.1%;and 0.3% tetrazolium solution for 6;18 and 24 hours at 35°C, in the dark. The evaluation was performed on the basis of stain intensity and aspect of the tissues. Based on color patterns, the seed images were classified in eight viability classes. Those results were compared with the germination test in a laboratory. The treatment in concentration of 0.1% for 6 hours was considered adequate for the specie, considering the adequate staining and time for analysis.

关 键 词：Forest Seeds Fava-Arara-Tucupi VIABILITY AMAZON 

分 类 号：R73[医药卫生—肿瘤]