Seed Storability of CIMMYT Core Wheat Germplasm Panel and Their Haplotypes in <i>Lipoxygenase</i>Locus  

作　　者：Yumin Yang Jipeng Qu Jing Zhang Shijie Xiang Jun Li Hongshen Wan Yumin Yang;Jipeng Qu;Jing Zhang;Shijie Xiang;Jun Li;Hongshen Wan(Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Areas), Chengdu, China;Institute of Agricultural Resources and Environment, Sichuan Academy of Agricultural Sciences, Chengdu, China;Panxi Crops Research and Utilization Key Laboratory of Sichuan Province, Xichang University, Xichang, China;Food Safety Detection Key Laboratory of Sichuan, Technology Center of Chengdu Customs, Chengdu, China;Sichuan Agricultural products Quality and Safety Center, Chengdu, China;Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China)

机构地区：[1]Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Areas), Chengdu, China [2]Institute of Agricultural Resources and Environment, Sichuan Academy of Agricultural Sciences, Chengdu, China [3]Panxi Crops Research and Utilization Key Laboratory of Sichuan Province, Xichang University, Xichang, China [4]Food Safety Detection Key Laboratory of Sichuan, Technology Center of Chengdu Customs, Chengdu, China [5]Sichuan Agricultural products Quality and Safety Center, Chengdu, China [6]Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China

出　　处：《Agricultural Sciences》2021年第9期977-989,共13页农业科学（英文）

摘　　要：Seed storability (SS), also called seed longevity, is a valuable trait for seed banks and providing reliable crop seeds to farmers, which is usually negatively correlated to lipoxygenase (LOX) activity. In this study, the seed storability of 60 accessions of CIMMYT core wheat germplasm panel (CIMCOG) was investigated through artificial aging (AA) test, including three parameters relative germination potential (RGP), relative germination rate (RGR) and relative seedling vigor index (RVI). Significant positive relationships were observed among RGP, RGR and RVI. And the genotypes at three LOX activity related QTLs/genes <em>QLpx.caas</em>-4<em>B</em>, <em>QLpx.caas</em>-1<em>AL</em> and <em>TaLOX</em>-<em>B</em>1 were also identified with published trait-associated molecular markers. For <em>QLpx.caas</em>-4<em>B</em>, a total of five alleles were detected at the locus of <em>Xgwm</em>251, and one marker-trait association was identified for RVI. Four and two alleles were detected at the loci of <em>QLpx.caas</em>-1<em>AL</em> and <em>TaLoxB</em>1 that were significantly associated with RGP, RGR and RVI, respectively. A total of 9 haplotypes were detected at three lipoxygenase activity related gene loci, and the haplotype of three lipoxygenase loci showed a significant association with RGP, RGR and RVI. The haplotype of <em>Xgwm</em>251_{-125<em>bp</em>}+ <em>Xwmc</em>312_{-247<em>bp</em>} + <em>TaLox</em>-<em>B</em>1<em>b</em> produced seeds with the best storability in the CIMCOG, which could benefit the breeding for wheat with good seed storability.Seed storability (SS), also called seed longevity, is a valuable trait for seed banks and providing reliable crop seeds to farmers, which is usually negatively correlated to lipoxygenase (LOX) activity. In this study, the seed storability of 60 accessions of CIMMYT core wheat germplasm panel (CIMCOG) was investigated through artificial aging (AA) test, including three parameters relative germination potential (RGP), relative germination rate (RGR) and relative seedling vigor index (RVI). Significant positive relationships were observed among RGP, RGR and RVI. And the genotypes at three LOX activity related QTLs/genes <em>QLpx.caas</em>-4<em>B</em>, <em>QLpx.caas</em>-1<em>AL</em> and <em>TaLOX</em>-<em>B</em>1 were also identified with published trait-associated molecular markers. For <em>QLpx.caas</em>-4<em>B</em>, a total of five alleles were detected at the locus of <em>Xgwm</em>251, and one marker-trait association was identified for RVI. Four and two alleles were detected at the loci of <em>QLpx.caas</em>-1<em>AL</em> and <em>TaLoxB</em>1 that were significantly associated with RGP, RGR and RVI, respectively. A total of 9 haplotypes were detected at three lipoxygenase activity related gene loci, and the haplotype of three lipoxygenase loci showed a significant association with RGP, RGR and RVI. The haplotype of <em>Xgwm</em>251_{-125<em>bp</em>}+ <em>Xwmc</em>312_{-247<em>bp</em>} + <em>TaLox</em>-<em>B</em>1<em>b</em> produced seeds with the best storability in the CIMCOG, which could benefit the breeding for wheat with good seed storability.

关 键 词：Wheat Seed Storability Lipoxygenase Trait-Marker Association HAPLOTYPE 

分 类 号：S51[农业科学—作物学]