Inteins--A Focus on the Biotechnological Applications of Splicing-Promoting Proteins  

作　　者：Manfredi Miraula Charmaine Enculescu Gerhard Schenk Natasa Mitic 

机构地区：[1]Department of Chemistry,Maynooth University,Maynooth,Ireland [2]School of Molecular and Microbial Biosciences,The University of Queensland,St.Lucia,Queensland,Australia

出　　处：《American Journal of Molecular Biology》2015年第2期42-56,共15页美国分子生物学期刊（英文）

基　　金：MM would like to thank the Science Foundation Ireland(SFI)for a postgraduate scholarship received via a President of Ireland Young Researcher Award(PIYRA)bestowed on NM;GS acknowledges the award of a Future Fellowship from the Australian Research Council.

摘　　要：The main aim of this mini-review is to illustrate strategies and industrial applications based on inteins (INTErnal proteINS), which belong to a class of autocatalytic enzymes that are able to perform a catalytic reaction on a single substrate. However, since practical applications of inteins are strongly guided by a detailed understanding of their biological mechanisms and functions, the first part of this review will thus briefly discuss the physiological roles of inteins, describing what is currently known about their mechanisms of action. In the second part, specific biotechnological applications of inteins will be outlined (i.e. their use for (i) the purification of recombinant proteins, (ii) the cyclization of proteins and (iii) the production of seleno-proteins), paying attention to both potential strengths and weaknesses of this technology.The main aim of this mini-review is to illustrate strategies and industrial applications based on inteins (INTErnal proteINS), which belong to a class of autocatalytic enzymes that are able to perform a catalytic reaction on a single substrate. However, since practical applications of inteins are strongly guided by a detailed understanding of their biological mechanisms and functions, the first part of this review will thus briefly discuss the physiological roles of inteins, describing what is currently known about their mechanisms of action. In the second part, specific biotechnological applications of inteins will be outlined (i.e. their use for (i) the purification of recombinant proteins, (ii) the cyclization of proteins and (iii) the production of seleno-proteins), paying attention to both potential strengths and weaknesses of this technology.

关 键 词：INTEIN Protein Purification Tagged Protein CYCLIZATION SELENOPROTEIN 

分 类 号：R73[医药卫生—肿瘤]