ESBL Production and Multidrug Resistance of Salmonella Serovars Isolates in Benue State  

作　　者：B. O. Okpa G. M. Gberikon C. U. Aguoru I. O. Ogbonna B. O. Okpa;G. M. Gberikon;C. U. Aguoru;I. O. Ogbonna(Department of Microbiology, Federal University of Agriculture, Makurdi, Nigeria)

机构地区：[1]Department of Microbiology, Federal University of Agriculture, Makurdi, Nigeria

出　　处：《American Journal of Molecular Biology》2020年第3期200-223,共24页美国分子生物学期刊（英文）

摘　　要：Studies on ESBL-producing and multi-drug resistance of <em>Salmonella</em> serovars distributed in Benue State were investigated. A total of four hundred and twenty (420) clinical stool samples, seventy (70) from each local government area were randomly collected from selected hospitals and analyzed for the presence of <em>Salmonella</em> spp. The isolates were characterized using Gram staining and biochemical tests. The result of AP120E biochemical test strip which contained dehydrated bacterial media and biochemical reagents in twenty (20) separate compartments. The result was obtained by evaluation of the compartments due to observed changes in after 24 hours where others were read by adding up reagents (Ferric chloride, Kovacs V.P reagents). The results were analyzed afterwards in accordance with the manufacturer’s software and positive results with ≥89% potential were confirmed as <em>Salmonella</em> spp. Amplified plasmids derived from 18 <em>Salmonella</em> strains recognized were made up of 23,130 base pairs. ESBL (Extended-spectrum beta-lactamases) genes were located on the plasmids. Two of the ESBL genes found were TEM genes and CTX-M (415 bp). Strains such as <em>S. enterica</em> Typhimurium-CP014981.1, <em>S. enterica</em> Enteritidis-CP007325.2, <em>S. enterica</em> Typhimurium-CP024619.1, <em>S. enterica</em> Typhimurium-CP023166.1, <em>S. bongori-FR877557</em> and <em>S. enterica</em> Enteritidis-TY1 possessed TEM genes where as <em>S. enterica</em> Heidelberg-CP019176.1, <em>S. enterica</em> Typhi-AL513382.1 and <em>S. enterica</em> Typhimurium-MH196335.1 possessed CTX-M. Antibiotic resistance testing was performed using Kirby-Bauer disc diffusion method. The overall percentage susceptibility of the eight antibiotics tested on <em>Salmonella</em> serovars isolates shows that GEN had the highest % susceptibility of 100% followed by NIT (72.2%) and COT (66.7%) before and after plasmid curing. % susceptibility was lower before curing than after curing in CXC, CHL and TET. It was low (5.6%) in ERYStudies on ESBL-producing and multi-drug resistance of <em>Salmonella</em> serovars distributed in Benue State were investigated. A total of four hundred and twenty (420) clinical stool samples, seventy (70) from each local government area were randomly collected from selected hospitals and analyzed for the presence of <em>Salmonella</em> spp. The isolates were characterized using Gram staining and biochemical tests. The result of AP120E biochemical test strip which contained dehydrated bacterial media and biochemical reagents in twenty (20) separate compartments. The result was obtained by evaluation of the compartments due to observed changes in after 24 hours where others were read by adding up reagents (Ferric chloride, Kovacs V.P reagents). The results were analyzed afterwards in accordance with the manufacturer’s software and positive results with ≥89% potential were confirmed as <em>Salmonella</em> spp. Amplified plasmids derived from 18 <em>Salmonella</em> strains recognized were made up of 23,130 base pairs. ESBL (Extended-spectrum beta-lactamases) genes were located on the plasmids. Two of the ESBL genes found were TEM genes and CTX-M (415 bp). Strains such as <em>S. enterica</em> Typhimurium-CP014981.1, <em>S. enterica</em> Enteritidis-CP007325.2, <em>S. enterica</em> Typhimurium-CP024619.1, <em>S. enterica</em> Typhimurium-CP023166.1, <em>S. bongori-FR877557</em> and <em>S. enterica</em> Enteritidis-TY1 possessed TEM genes where as <em>S. enterica</em> Heidelberg-CP019176.1, <em>S. enterica</em> Typhi-AL513382.1 and <em>S. enterica</em> Typhimurium-MH196335.1 possessed CTX-M. Antibiotic resistance testing was performed using Kirby-Bauer disc diffusion method. The overall percentage susceptibility of the eight antibiotics tested on <em>Salmonella</em> serovars isolates shows that GEN had the highest % susceptibility of 100% followed by NIT (72.2%) and COT (66.7%) before and after plasmid curing. % susceptibility was lower before curing than after curing in CXC, CHL and TET. It was low (5.6%) in ERY

关 键 词：ESBL SALMONELLA SEROVARS SUSCEPTIBILITY 

分 类 号：R44[医药卫生—诊断学]