Incidence of Oxa23 and Oxa51 Genes Associated with Bacterial Isolated from Patients with Urosepsis: Single Centre Prespective  

作　　者：Salma Omer Ibrahim Elimam M. A. Mohammed Sami Mahjoub Taha Sanaa Mohammed Yousif Hajir Omer Omer Omer Mirghani Seif-Elnasr Seitelbanat Yassin Yousif Abdelhameed Mohammed Omer Abu Elhasan Mohamed Taj-Eldin Adam Dawoud Abakar Elhadi Abdalla Ahmed Salaheldein G. Elzaki Salma Omer Ibrahim;Elimam M. A. Mohammed;Sami Mahjoub Taha;Sanaa Mohammed Yousif;Hajir Omer;Omer Omer;Mirghani Seif-Elnasr;Seitelbanat Yassin;Yousif Abdelhameed Mohammed;Omer Abu Elhasan;Mohamed Taj-Eldin;Adam Dawoud Abakar;Elhadi Abdalla Ahmed;Salaheldein G. Elzaki(Department of Medical Microbiology, Faculty of Medical Lab Sciences, University of Gezira, Wad Medani, Sudan;Department of Urology, Faculty of Medicine, University of Gezira, Wad Medani, Sudan;National Blood Bank, Ministry of Health, Khartoum, Sudan;University Hospital Galway, Galway, Ireland;Department of Urology, Gezira Hospital for Renal Disease and Surgery, Wad Medani, Sudan;Department of Pharmaceutics, Faculty of Pharmacy, University of Gezira, Wad Medani, Sudan;Department of Clinical Chemistry, National Cancer Institute, University of Gezira, Wad Medani, Sudan;Department of Medical Microbiology, Wad Medani College for Medical Science and Technology, Wad Medani, Sudan;Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan;Department of Epidemiology, Tropical Medicine Research Institute, Khartoum, Sudan)

机构地区：[1]Department of Medical Microbiology, Faculty of Medical Lab Sciences, University of Gezira, Wad Medani, Sudan [2]Department of Urology, Faculty of Medicine, University of Gezira, Wad Medani, Sudan [3]National Blood Bank, Ministry of Health, Khartoum, Sudan [4]University Hospital Galway, Galway, Ireland [5]Department of Urology, Gezira Hospital for Renal Disease and Surgery, Wad Medani, Sudan [6]Department of Pharmaceutics, Faculty of Pharmacy, University of Gezira, Wad Medani, Sudan [7]Department of Clinical Chemistry, National Cancer Institute, University of Gezira, Wad Medani, Sudan [8]Department of Medical Microbiology, Wad Medani College for Medical Science and Technology, Wad Medani, Sudan [9]Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Wad Medani, Sudan [10]Department of Epidemiology, Tropical Medicine Research Institute, Khartoum, Sudan

出　　处：《American Journal of Molecular Biology》2022年第3期85-96,共12页美国分子生物学期刊（英文）

摘　　要：Background: Urosepsis is one of the most common infections that require empirical broad spectrum antibiotics immediately after diagnosis. This has led to development of bacterial resistance by acquiring the capability to destroy the β-lactam ring. Methodology: This is a cross-sectional hospital-based study. The study was conducted from 2019 to 2020 at Gezira Hospital for Renal diseases and surgery (GHRDS). A hundred patients were diagnosed clinically with urosepsis and the isolated organisms were Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumonia and Pseudomonas aeruginosa. The susceptibility test was conducted by Kirby Bauer disc diffusion technique according to clinical laboratory standard institute (CLSI) guidelines. Seventy eight samples of bacterial genomic DNA were confirmed by 16srRNA and multiplex PCR, were performed for genotypic blaOXA-51 and blaOXA-23 gene characterization of isolated bacteria. Then gel electrophoresis was used to identify the presence or absence of (blaOXA-51 and blaOXA-23) genes. Results: 88.5% (69/78) in 16srRNA detected. Using multiplex PCR, the frequencies of blaOXA-51 and blaOXA-23 genes were 13% and 10.1%, respectively. The percentages of isolates which yielded both blaOXA-51 and blaOXA-23 among P. aeruginosa was 25% (1/4), among K. pneumonia was 17% (1/6), and among E. coli was 8% (3/37). Only blaOXA-51 was detected in P. mirabilis 10% (1/10) and only blaOXA-23 was detected in S. aureus 5% (1/18). Conclusion: In this study, the presence of blaOXA-51 and blaOXA-23 genes was increased in the isolated bacteria.Background: Urosepsis is one of the most common infections that require empirical broad spectrum antibiotics immediately after diagnosis. This has led to development of bacterial resistance by acquiring the capability to destroy the β-lactam ring. Methodology: This is a cross-sectional hospital-based study. The study was conducted from 2019 to 2020 at Gezira Hospital for Renal diseases and surgery (GHRDS). A hundred patients were diagnosed clinically with urosepsis and the isolated organisms were Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumonia and Pseudomonas aeruginosa. The susceptibility test was conducted by Kirby Bauer disc diffusion technique according to clinical laboratory standard institute (CLSI) guidelines. Seventy eight samples of bacterial genomic DNA were confirmed by 16srRNA and multiplex PCR, were performed for genotypic blaOXA-51 and blaOXA-23 gene characterization of isolated bacteria. Then gel electrophoresis was used to identify the presence or absence of (blaOXA-51 and blaOXA-23) genes. Results: 88.5% (69/78) in 16srRNA detected. Using multiplex PCR, the frequencies of blaOXA-51 and blaOXA-23 genes were 13% and 10.1%, respectively. The percentages of isolates which yielded both blaOXA-51 and blaOXA-23 among P. aeruginosa was 25% (1/4), among K. pneumonia was 17% (1/6), and among E. coli was 8% (3/37). Only blaOXA-51 was detected in P. mirabilis 10% (1/10) and only blaOXA-23 was detected in S. aureus 5% (1/18). Conclusion: In this study, the presence of blaOXA-51 and blaOXA-23 genes was increased in the isolated bacteria.

关 键 词：UROSEPSIS Carbapenem-Resistant Enterobacteriaceae (CRE) blaOXA-51 and blaOXA-23 

分 类 号：R73[医药卫生—肿瘤]