Comparative Performance of Microscopy and Nested PCR for the Detection of Cryptosporidium Species in Patients Living with HIV/AIDS in Abidjan (Côte d’Ivoire)  

作　　者：N’Docho Assi Fiacre-Tanguy Gonédélé Bi Sery Ernest Tuo Karim Bialé Marina Danielle Datcho-Gouenet Ayaud N’Guessan Tiacoh Landry Koffi Attoungbré Clément Koffi David Ako Ako Aristide Bérenger Vanga Bosson Abo Henriette Touré Offianan André N’Docho Assi Fiacre-Tanguy;Gonédélé Bi Sery Ernest;Tuo Karim;Bialé Marina Danielle Datcho-Gouenet Ayaud;N’Guessan Tiacoh Landry;Koffi Attoungbré Clément;Koffi David;Ako Ako Aristide Bérenger;Vanga Bosson Abo Henriette;Touré Offianan André(Department of Parasitology Mycology, Institut Pasteur, Abidjan, Côte d’Ivoire;Laboratory of Genetics and Species Improvement, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire;Department of Parasitology Mycology, UFR Pharmaceutical and Biological Sciences, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire)

机构地区：[1]Department of Parasitology Mycology, Institut Pasteur, Abidjan, Cô te d’Ivoire [2]Laboratory of Genetics and Species Improvement, Félix Houphouë t-Boigny University, Abidjan, Cô te d’Ivoire [3]Department of Parasitology Mycology, UFR Pharmaceutical and Biological Sciences, Félix Houphouë t-Boigny University, Abidjan, Cô te d’Ivoire

出　　处：《American Journal of Molecular Biology》2023年第1期18-31,共14页美国分子生物学期刊（英文）

摘　　要：Cryptosporidium spp. infection is one of the causes of diarrhea in people living with HIV/AIDS. The objective of this study is to compare the sensitivity of microscopy and molecular biology to determine the prevalence of Cryptosporidium spp. in Patients Living With HIV (PLWH). This is a descriptive cross-sectional study conducted in three care centers for people living with HIV/AIDS in Abidjan. It took place from November 2018 to March 2020. Sociodemographic data were obtained via a questionnaire. Stool and blood samples were collected and analyzed for microscopy and Nested PCR detection of Cryptosporidium spp. Blood samples were analyzed for CD4+ count. A total of 363 stool samples were collected from the three sites. Individuals aged 40 - 50 years (36.52%) were most likely to participate in the study. HIV Type 1 accounted for 86.22% of the study population. The samples collected consisted of 47.65% diarrheal stool. Microscopic examination of the stool yielded a prevalence of 3.86% for Cryptosporidium spp. while the prevalence was 3.96% with molecular identification. No statistically significant difference was observed between these two prevalences (χ² = 0.26;p = 0.609). CD4+ count was the factor associated with Cryptosporidium spp. infection for both microscopy (OR = 0.887, p = 0.001) and PCR (OR = 0.896, p = 0.001). This study demonstrated that Nested PCR improves the detection of Cryptosporidium spp. in patient diagnosis.Cryptosporidium spp. infection is one of the causes of diarrhea in people living with HIV/AIDS. The objective of this study is to compare the sensitivity of microscopy and molecular biology to determine the prevalence of Cryptosporidium spp. in Patients Living With HIV (PLWH). This is a descriptive cross-sectional study conducted in three care centers for people living with HIV/AIDS in Abidjan. It took place from November 2018 to March 2020. Sociodemographic data were obtained via a questionnaire. Stool and blood samples were collected and analyzed for microscopy and Nested PCR detection of Cryptosporidium spp. Blood samples were analyzed for CD4+ count. A total of 363 stool samples were collected from the three sites. Individuals aged 40 - 50 years (36.52%) were most likely to participate in the study. HIV Type 1 accounted for 86.22% of the study population. The samples collected consisted of 47.65% diarrheal stool. Microscopic examination of the stool yielded a prevalence of 3.86% for Cryptosporidium spp. while the prevalence was 3.96% with molecular identification. No statistically significant difference was observed between these two prevalences (χ² = 0.26;p = 0.609). CD4+ count was the factor associated with Cryptosporidium spp. infection for both microscopy (OR = 0.887, p = 0.001) and PCR (OR = 0.896, p = 0.001). This study demonstrated that Nested PCR improves the detection of Cryptosporidium spp. in patient diagnosis.

关 键 词：CRYPTOSPORIDIUM PCR MICROSCOPY PLHIV Côte D’Ivoire 

分 类 号：R51[医药卫生—内科学]