Molecular Genotyping of Human Papillomavirus (HPV) in Hypopharyngeal Cancer  

作　　者：Babacar Faye Soukeynatou Diabira Omar Bassoum Doudou G. M. Niang Malick Ndiaye Baye Karim Diallo Alioune Dièye Babacar Faye;Soukeynatou Diabira;Omar Bassoum;Doudou G. M. Niang;Malick Ndiaye;Baye Karim Diallo;Alioune Dièye(Laboratory of Molecular Biology, Military Hospital of Ouakam (HMO), Dakar, Senegal;AIDS Program of the Senegalese Armed Forces, Military Hospital of Ouakam (HMO), Dakar, Senegal;Service of Molecular Biology, Department of Pharmacy, Faculty of Medicine, Pharmacy and Odonto-Stomatology of the Cheikh Anta Diop University of Dakar, Dakar, Senegal;Public Health Unit, Faculty of Medecine, Pharmacy and Odonto-Stomatology of Cheikh Anta Diop University of Dakar, Dakar, Senegal;Service of Immunology, Department of Pharmacy, Faculty of Medicine, Pharmacy and Odonto-Stomatology of the Cheikh Anta Diop University of Dakar, Dakar, Senegal;Service of ORL, Fann Hospital, Dakar, Senegal)

机构地区：[1]Laboratory of Molecular Biology, Military Hospital of Ouakam (HMO), Dakar, Senegal [2]AIDS Program of the Senegalese Armed Forces, Military Hospital of Ouakam (HMO), Dakar, Senegal [3]Service of Molecular Biology, Department of Pharmacy, Faculty of Medicine, Pharmacy and Odonto-Stomatology of the Cheikh Anta Diop University of Dakar, Dakar, Senegal [4]Public Health Unit, Faculty of Medecine, Pharmacy and Odonto-Stomatology of Cheikh Anta Diop University of Dakar, Dakar, Senegal [5]Service of Immunology, Department of Pharmacy, Faculty of Medicine, Pharmacy and Odonto-Stomatology of the Cheikh Anta Diop University of Dakar, Dakar, Senegal [6]Service of ORL, Fann Hospital, Dakar, Senegal

出　　处：《American Journal of Molecular Biology》2023年第4期227-240,共14页美国分子生物学期刊（英文）

摘　　要：Background: In recent years, head and neck cancers have become common worldwide, ranking sixth in incidence. In 2007, in France the incidence increased by 14,697 including 11,158 among men, which places them in fourth place. The same year, 32,268 patients were hospitalized for this pathology, but 95% are associated with alcohol and tobacco poisoning. Few data exist on these cancers in Africa and Senegal. In recent years, many studies have hypothesized that about 25% of head and neck cancers are associated with high-risk oncogenic human papillomaviruses (HPV) whose role in cervical cancer was already widely established. Objective: To know the prevalence and genotypes of HPV in head and neck cancers, particularly hypopharyngeal cancer. Material and method: This study was carried out on samples of biopsies of hypopharynx cancerous tissue (ulcerative-budding lesion) and healthy oropharyngeal tissue obtained from the ENT department of the Fann hospital, then sent to the Molecular Biology Unit of the Ouakam military hospital (HMO). The nucleic acids extraction was carried out using the standard method of the Zymo research kit “Quick-DNA^TM Miniprep Plus kit” https://www.zymoresearch.com/. Molecular HPV detection and genotyping were performed by multiplex RT-PCR with the Seegene Anyplex^TM II HPV28 kit Detection on a Biorad CFX96 automaton according to the manufacturer’s protocol for the simultaneous genotyping of 28 types of HPV including 19 at High Risk (HR) and 9 low risk (LR). Results: 156 patients were sampled, 61 Hypopharynx cancer biopsies and 95 healthy tissues. The median age of the general population was 36.5 years [12, 73];the median age of the population with hypopharyngeal cancer of 40 years. Of the general study population 24.36% (38/156) was infected with HPV. In populations with hypopharyngeal cancer, HPV prevalence was 19.67% (12/61), 17.84% (5/28) in men and 21.21% (7/33) in women. HPV6 was the most frequently encountered genotype in the cancer population. Multiple infections Background: In recent years, head and neck cancers have become common worldwide, ranking sixth in incidence. In 2007, in France the incidence increased by 14,697 including 11,158 among men, which places them in fourth place. The same year, 32,268 patients were hospitalized for this pathology, but 95% are associated with alcohol and tobacco poisoning. Few data exist on these cancers in Africa and Senegal. In recent years, many studies have hypothesized that about 25% of head and neck cancers are associated with high-risk oncogenic human papillomaviruses (HPV) whose role in cervical cancer was already widely established. Objective: To know the prevalence and genotypes of HPV in head and neck cancers, particularly hypopharyngeal cancer. Material and method: This study was carried out on samples of biopsies of hypopharynx cancerous tissue (ulcerative-budding lesion) and healthy oropharyngeal tissue obtained from the ENT department of the Fann hospital, then sent to the Molecular Biology Unit of the Ouakam military hospital (HMO). The nucleic acids extraction was carried out using the standard method of the Zymo research kit “Quick-DNA^TM Miniprep Plus kit” https://www.zymoresearch.com/. Molecular HPV detection and genotyping were performed by multiplex RT-PCR with the Seegene Anyplex^TM II HPV28 kit Detection on a Biorad CFX96 automaton according to the manufacturer’s protocol for the simultaneous genotyping of 28 types of HPV including 19 at High Risk (HR) and 9 low risk (LR). Results: 156 patients were sampled, 61 Hypopharynx cancer biopsies and 95 healthy tissues. The median age of the general population was 36.5 years [12, 73];the median age of the population with hypopharyngeal cancer of 40 years. Of the general study population 24.36% (38/156) was infected with HPV. In populations with hypopharyngeal cancer, HPV prevalence was 19.67% (12/61), 17.84% (5/28) in men and 21.21% (7/33) in women. HPV6 was the most frequently encountered genotype in the cancer population. Multiple infections

关 键 词：HPV GENOTYPES RT-PCR ENT Cancer HYPOPHARYNX 

分 类 号：R73[医药卫生—肿瘤]