Genotypic Profiling and Clinical Impact of Helicobacter pylori Virulence Genes (GLM, HPU, VacA, CagA, and IceA) in Gastroduodenal Diseases among Libyan Patients  

作　　者：Aya M. Al-Farjany Madiha W. El-Awamie Nariman A. Elsharif Omar S. Alqabbasi Wail A. Alhawari Fatema I. E. Najem Tarek S. Bader Farag A. Bleiblo Aya M. Al-Farjany;Madiha W. El-Awamie;Nariman A. Elsharif;Omar S. Alqabbasi;Wail A. Alhawari;Fatema I. E. Najem;Tarek S. Bader;Farag A. Bleiblo(Department of Life Sciences/Microbiology Programme, School of Basic Sciences, Libyan Academy of Postgraduate Studies, Benghazi, Libya;Department of Microbiology, Faculty of Science, University of Benghazi, Benghazi, Libya;Department of Molecular Diagnosis, Faculty of Biomedical Sciences, University of Benghazi, Benghazi, Libya;Libyan International Medical University, Benghazi, Libya;Benghazi Center for Infectious Diseases and Immunology, Benghazi, Libya;Navco Pharmaceuticals Limited, Burlington, Ontario, Canada)

机构地区：[1]Department of Life Sciences/Microbiology Programme, School of Basic Sciences, Libyan Academy of Postgraduate Studies, Benghazi, Libya [2]Department of Microbiology, Faculty of Science, University of Benghazi, Benghazi, Libya [3]Department of Molecular Diagnosis, Faculty of Biomedical Sciences, University of Benghazi, Benghazi, Libya [4]Libyan International Medical University, Benghazi, Libya [5]Benghazi Center for Infectious Diseases and Immunology, Benghazi, Libya [6]Navco Pharmaceuticals Limited, Burlington, Ontario, Canada

出　　处：《American Journal of Molecular Biology》2025年第1期53-74,共22页美国分子生物学期刊(英文)

摘　　要：Helicobacter pylori infection represents a widespread chronic condition with varying prevalence influenced by race, ethnicity, and geography. The severity of H. pylori-associated diseases is determined by an array of virulence factors. Although extensive studies have been conducted globally, data on the distribution of Helicobacter pylori virulence genes in Libya remain limited, constraining insights into the pathogenicity of local strains and hindering the development of targeted interventions. This study aimed to evaluate the prevalence of H. pylori infection, characterize essential virulence genes [vacA variants (s1/s2, m1/m2), cagA, and iceA1], and examine their association with gastroduodenal diseases among Libyan patients. Gastric biopsies from 144 participants were analyzed using polymerase chain reaction (PCR) assays, and risk factor data were collected via questionnaires. H. pylori was detected in 63.2% of samples by PCR. The vacA gene was present in 84.6% of cases, cagA in 58.2%, and iceA1 in 29.7%. Among vacA variants, s1 allele was most common (53.2%), followed by m1 (42.9%), m2 (37.7%), and s2 (13%) alleles. Significant associations were identified between specific virulence genes and the development of gastroduodenal diseases, highlighting their role in pathogenicity. This investigation is one of Libya’s first comprehensive assessments of H. pylori virulence factors, addressing a critical epidemiological gap. The high prevalence of virulence genes suggests their potential as disease biomarkers. These findings contribute to a deeper understanding of H. pylori pathogenicity within the Libyan population and establish a basis for future clinical interventions and public health strategies to manage and prevent H. pylori-associated diseases in Libya and comparable regions.Helicobacter pylori infection represents a widespread chronic condition with varying prevalence influenced by race, ethnicity, and geography. The severity of H. pylori-associated diseases is determined by an array of virulence factors. Although extensive studies have been conducted globally, data on the distribution of Helicobacter pylori virulence genes in Libya remain limited, constraining insights into the pathogenicity of local strains and hindering the development of targeted interventions. This study aimed to evaluate the prevalence of H. pylori infection, characterize essential virulence genes [vacA variants (s1/s2, m1/m2), cagA, and iceA1], and examine their association with gastroduodenal diseases among Libyan patients. Gastric biopsies from 144 participants were analyzed using polymerase chain reaction (PCR) assays, and risk factor data were collected via questionnaires. H. pylori was detected in 63.2% of samples by PCR. The vacA gene was present in 84.6% of cases, cagA in 58.2%, and iceA1 in 29.7%. Among vacA variants, s1 allele was most common (53.2%), followed by m1 (42.9%), m2 (37.7%), and s2 (13%) alleles. Significant associations were identified between specific virulence genes and the development of gastroduodenal diseases, highlighting their role in pathogenicity. This investigation is one of Libya’s first comprehensive assessments of H. pylori virulence factors, addressing a critical epidemiological gap. The high prevalence of virulence genes suggests their potential as disease biomarkers. These findings contribute to a deeper understanding of H. pylori pathogenicity within the Libyan population and establish a basis for future clinical interventions and public health strategies to manage and prevent H. pylori-associated diseases in Libya and comparable regions.

关 键 词：H. pylori Virulence Genes Gastroduodenal Diseases Gastric Biopsy PCR 

分 类 号：R57[医药卫生—消化系统]