Leaf Anatomy of Prosthechea moojenii(Pabst)W.E.Higgins and P.silvana Cath.&V.P.Castro(Orchidaceae)as an Approach to Taxonomy of Species Occurring in Biomes Cerrado and Atlantic Forest of the Bahia  

作　　者：Filipe de Castro Seixas Silvana Helena Nascimento Monteiro Lazaro Benedito da Silva Kelly Regina Batista Leite 

机构地区：[1]Instituto de Biologia,Departamento de Botanica,Laboratório de Anatomia Vegetal e Identificacao de Madeiras,Campus Univer-sitário de Ondina,Universidade Federal da Bahia,Salvador,Brasil [2]Instituto de Pesquisa Jardim Botanico do Rio de Janeiro,Uni-dade de Botanica Sistemática,Rio de Janeiro,Brasil

出　　处：《American Journal of Plant Sciences》2012年第12期1752-1758,共7页美国植物学期刊（英文）

基　　金：thank FAPESB-Fundacao de AmparoàPesquisa do Estado da Bahia for the financial support for the development of this research(PPP 083/2010 and PNE 0020/2011).

摘　　要：Prosthechea is a neotropical genus, belonging to the family Orchidaceae. The Brazil recorded 32 species of the genus of which nine occur in the state of Bahia, including P. silvana and P. moojenii. The first, P. silvana is epiphytic, endemic Atlantic Forest areas of South and Southeast of the Bahia and morphologically similar to P. moojenii, rupicolous species considered endemic to the Chapada Diamantina. There are doubts whether these two species are really distinct, because they differ only as to the way of life and aspects related to the size of the inflorescence and floral structure. Thus the anatomical study of these two species, emerges as a tool that aims to investigate the delimitation of these taxa. For this study, the sheet was cut freehand with a razor blade to obtain cross sections and treated by chemical means to obtain paradermic sections, all the material was stained with safranine/Astra blue (safrablau) and safranin, respectively, and the sections were then mounted between a semipermanent and cover slipped slides. The results allowed to distinguish P. silvana of the P. moojenii through the following characteristics observed in the leaf (thickening of the cell walls of epidermal cells, amount and disposition of sclerenchyma fibers and fiber arrangement of vascular and vascular bundles in the mesophyll) and root (number of arcs of xylem and phloem, thickening of cells median cortex), infers taxonomic characters for the genus. The data indicated anatomical distinction between the two species studied here of the Prosthechea, however, suggest the anatomical analysis of other organs to reproductive and vegetative.Prosthechea is a neotropical genus, belonging to the family Orchidaceae. The Brazil recorded 32 species of the genus of which nine occur in the state of Bahia, including P. silvana and P. moojenii. The first, P. silvana is epiphytic, endemic Atlantic Forest areas of South and Southeast of the Bahia and morphologically similar to P. moojenii, rupicolous species considered endemic to the Chapada Diamantina. There are doubts whether these two species are really distinct, because they differ only as to the way of life and aspects related to the size of the inflorescence and floral structure. Thus the anatomical study of these two species, emerges as a tool that aims to investigate the delimitation of these taxa. For this study, the sheet was cut freehand with a razor blade to obtain cross sections and treated by chemical means to obtain paradermic sections, all the material was stained with safranine/Astra blue (safrablau) and safranin, respectively, and the sections were then mounted between a semipermanent and cover slipped slides. The results allowed to distinguish P. silvana of the P. moojenii through the following characteristics observed in the leaf (thickening of the cell walls of epidermal cells, amount and disposition of sclerenchyma fibers and fiber arrangement of vascular and vascular bundles in the mesophyll) and root (number of arcs of xylem and phloem, thickening of cells median cortex), infers taxonomic characters for the genus. The data indicated anatomical distinction between the two species studied here of the Prosthechea, however, suggest the anatomical analysis of other organs to reproductive and vegetative.

关 键 词：Laeliinae Flora of Bahia Velamen ENDEMISM 

分 类 号：R73[医药卫生—肿瘤]